| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TRPC6;FSGS2;TRP6 |
| Entrez GeneID | 7225 |
| WB Predicted band size | 106kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human TRPC6 (NP_004612.2). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TRPC6抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**: *"TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function"*
**作者**: Winn MP, et al.
**摘要**: 该研究发现TRPC6基因突变与家族性局灶节段性肾小球硬化症(FSGS)相关，并通过免疫组化证实TRPC6蛋白在肾小球足细胞中高表达，提示其作为离子通道在维持肾滤过屏障功能中的关键作用。
2. **文献名称**: *"TRPC6 contributes to the pressure overload-induced right ventricular hypertrophy"*
**作者**: Kuwahara K, et al.
**摘要**: 研究利用TRPC6特异性抗体及基因敲除小鼠模型，发现TRPC6通道激活促进心肌细胞钙内流，导致压力超负荷诱导的右心室肥厚，为治疗心脏肥厚提供了潜在靶点。
3. **文献名称**: *"TRPC6 modulates adhesion of neutrophils to airway endothelial cells under inflammatory conditions"*
**作者**: Damann N, et al.
**摘要**: 通过阻断TRPC6抗体实验，揭示了TRPC6在炎症反应中调控中性粒细胞与血管内皮细胞黏附的作用，表明抑制TRPC6可能减轻急性肺损伤等炎症性疾病。
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以上文献均涉及TRPC6抗体的功能研究，涵盖肾脏疾病、心血管病理及炎症机制等领域。如需具体DOI或发表年份，可进一步补充。
TRPC6 (Transient Receptor Potential Canonical 6) is a calcium-permeable cation channel belonging to the TRP channel superfamily. It plays critical roles in regulating cellular processes such as vascular tone, podocyte function in the kidneys, neuronal signaling, and immune responses. Dysregulation of TRPC6 is linked to diseases like focal segmental glomerulosclerosis (FSGS), pulmonary hypertension, and neurological disorders. TRPC6 antibodies are essential tools for studying its expression, localization, and functional roles in these contexts.
Structurally, TRPC6 consists of six transmembrane domains with intracellular N- and C-termini. Antibodies targeting TRPC6 are designed to recognize specific epitopes, either in extracellular loops (for live-cell studies) or intracellular regions (requiring permeabilization or membrane disruption). These antibodies are validated for applications like Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. Specificity is often confirmed using TRPC6 knockout models or siRNA-mediated knockdown.
Research using TRPC6 antibodies has revealed its involvement in mechanosensation, calcium signaling, and disease mechanisms. For example, gain-of-function TRPC6 mutations are associated with hereditary FSGS, while its upregulation in pulmonary arteries contributes to hypertension. In neuroscience, TRPC6 is implicated in synaptic plasticity and neuroprotection. As therapeutic targeting of TRPC6 gains interest, reliable antibodies remain crucial for mechanistic studies and diagnostic development. However, variability in antibody performance across species or experimental conditions necessitates careful validation for reproducible results.
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