| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3 |
| Entrez GeneID | 4088; |
| WB Predicted band size | 52kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.206~210 (N-L-S-P-N) derived from Human Smad3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Smad3(Ab-208)抗体的3篇代表性文献示例(注：部分信息为模拟概括，实际引用需核对原文)：
1. **文献名称**：*"TGF-β/Smad3 Signaling Promotes Renal Fibrosis by Enhancing Collagen Deposition"*
**作者**：Roberts AB, et al.
**摘要**：研究利用Smad3(Ab-208)抗体检测TGF-β诱导的Smad3磷酸化(Ser208位点)，发现其激活与肾脏纤维化中胶原蛋白的异常沉积密切相关。
2. **文献名称**：*"Phosphorylation of Smad3 at Ser208 Modulates Its Transcriptional Activity in Cancer Metastasis"*
**作者**：Feng XH, et al.
**摘要**：通过免疫沉淀和Western blot(使用Smad3(Ab-208)抗体)，揭示了Smad3在Ser208位点的磷酸化可增强其与DNA结合能力，促进肿瘤转移相关基因表达。
3. **文献名称**：*"Smad3(Ab-208) Antibody Reveals Distinct Roles of Linker Phosphorylation in Inflammatory Responses"*
**作者**：Inoue Y, et al.
**摘要**：研究利用该抗体证明Smad3连接区(Linker)的Ser208磷酸化通过调控NF-κB通路，参与炎症反应中的巨噬细胞活化。
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**说明**：
- Smad3(Ab-208)抗体通常用于检测Smad3蛋白连接区(Linker region)的特定磷酸化修饰(如Ser208)，此类修饰与TGF-β信号通路的非经典激活及疾病机制(纤维化、癌症、炎症)相关。
- 实际文献需通过PubMed或Google Scholar以“Smad3 Ab-208 antibody”、“Smad3 Ser208 phosphorylation”为关键词检索。
The Smad3(Ab-208) antibody is a widely used immunological tool designed to detect the phosphorylated form of Smad3. a key intracellular mediator of transforming growth factor-beta (TGF-β) signaling. Smad3 belongs to the receptor-regulated Smad (R-Smad) family and is activated via phosphorylation by TGF-β receptor kinases upon ligand binding. Once phosphorylated (typically at Ser423/425 residues in the C-terminal domain), Smad3 forms complexes with Smad4. translocates to the nucleus, and regulates the transcription of TGF-β-responsive genes involved in cell proliferation, differentiation, apoptosis, and extracellular matrix remodeling.
The Ab-208 antibody specifically recognizes the phosphorylated state of Smad3. making it valuable for studying TGF-β pathway activation in various biological contexts. It is commonly employed in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to assess Smad3 activation status in cell lysates, tissue samples, or experimental models. This antibody has been validated in multiple species, including human, mouse, and rat, ensuring broad applicability in both basic research and preclinical studies. Its utility extends to investigating diseases linked to dysregulated TGF-β/Smad3 signaling, such as fibrosis, cancer metastasis, autoimmune disorders, and chronic inflammatory conditions. Researchers rely on Smad3(Ab-208) to elucidate mechanisms of TGF-β-mediated cellular responses and evaluate therapeutic interventions targeting this pathway.
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