Rabbit Polyclonal NFkB-p105(Ab-927) Antibody

The NF-κB-p105 (Ab-927) antibody is designed to detect the NF-κB1 protein, specifically targeting the phosphorylated form of p105 at serine 927 (Ser927). NF-κB (nuclear factor kappa B) is a family of transcription factors regulating immune responses, inflammation, cell survival, and proliferation. The NF-κB1 gene encodes p105. a precursor protein that undergoes limited proteasomal processing to generate the mature p50 subunit, a key component of NF-κB dimers. Phosphorylation at Ser927 in the C-terminal region of p105 is critical for its proteolytic processing into p50. a step tightly regulated by signaling pathways such as the IKK (IκB kinase) complex. This phosphorylation event facilitates the recruitment of E3 ubiquitin ligases, leading to partial degradation of the inhibitory C-terminal domain and release of active p50. The NF-κB-p105 (Ab-927) antibody is widely used in research to study NF-κB activation dynamics, particularly in contexts like inflammation, immune signaling, and cancer, where dysregulated NF-κB activity is common. It is validated for applications including Western blotting, immunoprecipitation, and immunohistochemistry, aiding in the detection of phosphorylated p105 in cell lines and tissue samples. Understanding p105 phosphorylation provides insights into the balance between canonical and non-canonical NF-κB pathways and their roles in disease mechanisms.