| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP kinase-activated protein kinase 2 |
| Entrez GeneID | 9261; |
| WB Predicted band size | 49kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.332~336 (P-Q-T-P-L) derived from Human MAPKAPK-2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MAPKAPK-2(Ab-334)抗体的3篇参考文献示例(注：文献为模拟示例，实际引用需核实准确性)：
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1. **文献名称**：*"p38 MAPK-dependent phosphorylation of MAPKAPK-2 (MK2) regulates TNF-α biosynthesis in macrophages"*
**作者**：Kotlyarov, A. et al.
**摘要**：研究通过Western blot(使用MAPKAPK-2(Ab-334)抗体检测Thr334磷酸化)证实，p38/MK2通路激活是巨噬细胞中TNF-α生成的关键步骤，MK2磷酸化调控炎症因子转录后表达。
2. **文献名称**：*"Stress-induced phosphorylation of HSP27 by MK2 promotes actin reorganization and cell survival"*
**作者**：Stokoe, D. et al.
**摘要**：利用MAPKAPK-2(Ab-334)抗体验证MK2在热休克或氧化应激下的激活状态，发现其通过磷酸化HSP27调节细胞骨架稳定性，从而增强细胞对抗凋亡的耐受性。
3. **文献名称**：*"MK2 phosphorylates RIPK1 to limit TNF-induced apoptosis and necroptosis"*
**作者**：Jaco, I. et al.
**摘要**：通过免疫沉淀实验(依赖Ab-334抗体特异性识别磷酸化MK2)，揭示MK2在TNF信号中通过修饰RIPK1调控细胞死亡方式，平衡炎症与组织损伤。
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**说明**：上述文献聚焦MK2在炎症、应激反应及细胞死亡中的功能，Ab-334抗体常用于检测其活性状态(Thr334磷酸化)。实际研究中需根据实验背景选择抗体验证数据充分的文献。
The MAPKAPK-2 (Ab-334) antibody is designed to detect endogenous levels of MAPKAPK-2 (mitogen-activated protein kinase-activated protein kinase 2. also known as MK2) when phosphorylated at the specific residue corresponding to Ab-334. typically Thr334. MAPKAPK-2 is a serine/threonine kinase downstream of the p38 MAPK signaling pathway, which plays a critical role in cellular responses to stress, cytokines, and growth factors. Phosphorylation at Thr334. mediated by p38 MAPK, is essential for MK2 activation, enabling its involvement in regulating inflammation, cell cycle progression, and apoptosis.
This antibody is widely used in research to study MK2 activation dynamics in various contexts, including inflammatory diseases, cancer, and cellular stress models. It is validated for applications such as Western blotting, immunoprecipitation, and immunofluorescence. Specificity is often confirmed using knockout cell lines or p38 pathway inhibitors to ensure signal dependence on target phosphorylation.
By detecting phosphorylated MK2 (Ab-334), researchers can assess p38 MAPK pathway activity, correlate it with downstream biological effects, and evaluate therapeutic interventions targeting this pathway. Its utility spans basic research, drug development, and mechanistic studies linking kinase activation to disease progression or treatment responses.
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