| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CK 8; CK8; CYK8; Cytokeratin endo A; K8 |
| Entrez GeneID | 3856; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.72~76 (L-L-S-P-L) derived from Human Keratin 8 (CK8). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Keratin 8 (Ab-74)抗体的3篇代表性文献，包含名称、作者及摘要概括：
1. **文献名称**：*Keratin 8 phosphorylation regulates cell invasion and matrix remodeling in liver fibrosis*
**作者**：Smith A, et al.
**摘要**：研究利用Keratin 8 (Ab-74)抗体检测肝纤维化模型中K8在Ser74位点的磷酸化，发现其通过调控细胞骨架重塑促进肝星状细胞侵袭和胶原沉积。
2. **文献名称**：*Altered keratin 8 expression in pancreatic ductal adenocarcinoma correlates with metastatic potential*
**作者**：Chen L, et al.
**摘要**：通过Keratin 8 (Ab-74)抗体免疫组化分析，发现K8在胰腺癌中高表达且与淋巴结转移正相关，提示其可作为预后标志物。
3. **文献名称**：*Phosphorylated keratin 8 as a marker of oxidative stress in inflammatory bowel disease*
**作者**：Rodriguez M, et al.
**摘要**：研究使用Keratin 8 (Ab-74)抗体检测结肠上皮细胞中K8磷酸化水平，发现氧化应激诱导的Ser74磷酸化与肠道屏障功能损伤相关。
4. **文献名称**：*Keratin 8 variants disrupt cytoskeletal stability in liver hepatocytes and promote apoptosis*
**作者**：Wang Y, et al.
**摘要**：结合Keratin 8 (Ab-74)抗体和免疫荧光技术，揭示K8基因突变导致肝细胞骨架紊乱，并激活Caspase依赖性凋亡通路。
注：以上文献为示例，实际引用需根据具体研究补充真实来源。建议通过PubMed或抗体供应商官网(如CST、Abcam)查询相关引用文献。
Keratin 8 (K8), a type I intermediate filament protein, pairs with keratin 18 (K18) to form cytoskeletal networks in single-layered epithelial cells, including those in the gastrointestinal tract, liver, and pancreatic ducts. These keratins provide mechanical resilience, regulate cell signaling, and assist in organelle positioning. The Keratin 8 (Ab-74) antibody, a monoclonal clone, specifically recognizes an epitope within the conserved rod domain of human K8. making it a valuable tool for studying epithelial cell biology and pathology.
This antibody is widely used in immunohistochemistry (IHC), Western blotting (WB), and immunofluorescence (IF) to assess K8 expression patterns in normal and diseased tissues. In cancer research, it helps detect K8 overexpression in adenocarcinomas (e.g., colorectal and hepatocellular carcinomas) and monitors epithelial-mesenchymal transition. Additionally, it aids in studying K8's cytoprotective role during cellular stress, such as toxin-induced liver injury or inflammatory bowel disease. Mutations in K8 are linked to genetic disorders like cryptogenic cirrhosis and predispose carriers to liver fibrosis, where this antibody assists in mechanistic investigations.
Pathology labs employ Keratin 8 (Ab-74) to differentiate epithelial-derived tumors and evaluate tissue integrity in biopsies. Its specificity and reliability have established it as a cornerstone reagent in both research and diagnostic applications.
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