| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Growth factor receptor bound protein 2-associated protein 1 |
| Entrez GeneID | 2549; |
| WB Predicted band size | 110kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around aa.625~629 (V-E-Y-L-D) derived from Human Gab1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下为3篇涉及Gab1 (phospho-Tyr627)抗体(如Ab-627克隆)应用的研究文献摘要概括：
---
1. **"Gab1 phosphorylation by PDGF receptor regulates endothelial cell migration and angiogenesis"**
*作者：Sakaguchi et al. (2008)*
摘要：研究通过PDGF受体激活Gab1的Tyr627磷酸化，使用特异性抗体(Ab-627)证实该位点磷酸化对血管内皮细胞迁移和血管生成的调控作用。
---
2. **"Role of Gab1 tyrosine phosphorylation in Met-mediated epithelial morphogenesis"**
*作者：Maroun et al. (2003)*
摘要：利用抗Gab1 pTyr627抗体(Ab-627)发现，Met受体激活后Gab1的Tyr627磷酸化是细胞形态发生和三维上皮结构形成的关键信号节点。
---
3. **"Gab1 sustains ERK activation and enhances mTORC1 signaling in hepatocellular carcinoma"**
*作者：Li et al. (2016)*
摘要：通过Western blot和免疫沉淀实验(使用Ab-627抗体)，揭示肝癌细胞中Gab1 Tyr627磷酸化通过ERK/mTOR通路促进肿瘤生长和耐药性。
---
注：实际文献中可能不会直接标注“Ab-627”克隆号，需根据抗体特性(如靶向pTyr627位点)匹配相关研究。建议通过抗体供应商(如CST、Abcam)官网的“参考文献”栏目获取准确引用信息。
The Gab1 (Grb2-associated binder 1) protein is a scaffolding adaptor molecule critical in mediating signal transduction pathways downstream of receptor tyrosine kinases (RTKs) and cytokine receptors. It belongs to the GAB family and contains multiple binding domains, including a pleckstrin homology (PH) domain, which facilitates membrane localization, and tyrosine phosphorylation sites that recruit downstream effectors like PI3K, SHP2. and Crk. Gab1 integrates signals from growth factors (e.g., EGF, HGF) and cytokines, regulating cellular processes such as proliferation, differentiation, migration, and apoptosis. Dysregulation of Gab1 is implicated in cancer, developmental disorders, and immune dysfunctions.
The Gab1 (Ab-627) antibody is a monoclonal antibody specifically designed to detect endogenous levels of Gab1 protein. It is widely used in applications like Western blotting, immunoprecipitation, and immunofluorescence to study Gab1 expression, phosphorylation status, and interaction partners in various biological contexts. Researchers employ this antibody to explore Gab1's role in signaling pathways (e.g., MAPK/ERK, PI3K/AKT) and its involvement in diseases, particularly cancer metastasis and resistance to therapies. The Ab-627 clone is valued for its specificity and reliability in distinguishing Gab1 from related family members (e.g., Gab2. Gab3), enabling precise mechanistic insights. Its utility extends to preclinical models, including cell lines and tissue samples, aiding in the validation of Gab1 as a therapeutic target or biomarker.
×
