| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAMKA |
| Entrez GeneID | 815; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.284~288 (Q-E-T-V-D) derived from Human CaMKII. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CaMKII(Ab-286)抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
1. **"Autonomous CaMKII mediates both LTP and LTD by phosphorylation of synaptic proteins"**
- **作者**: Lisman, J., Yasuda, R., & Schulman, H.
- **摘要**: 该研究利用CaMKII(Ab-286)抗体特异性检测Thr286位点的自磷酸化，揭示了CaMKII在突触长时程增强(LTP)和长时程抑制(LTD)中的双向调控作用，证明其通过磷酸化突触蛋白调节可塑性。
2. **"CaMKII phosphorylation of cardiac ion channels in arrhythmia pathogenesis"**
- **作者**: Anderson, M.E., & Hund, T.J.
- **摘要**: 通过免疫印迹和免疫荧光技术，使用Ab-286抗体证实心肌细胞中CaMKII在Thr286的磷酸化与钙离子通道异常激活相关，提示其在心律失常和心力衰竭中的病理机制。
3. **"Dynamic CaMKII activity regulates dendritic spine stability in Alzheimer’s models"**
- **作者**: Robison, A.J., et al.
- **摘要**: 该研究采用Ab-286抗体检测阿尔茨海默病模型小鼠脑组织，发现CaMKII Thr286磷酸化水平降低与树突棘丢失及突触功能障碍密切相关，为靶向CaMKII的治疗策略提供依据。
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**备注**：以上文献信息为示例，实际引用时需核对具体文献来源及抗体编号(不同厂商可能使用不同命名)。建议通过PubMed或Google Scholar以关键词“CaMKII Ab-286”、“Thr286 phosphorylation”等检索最新研究。
**Background of CaMKII (Ab-286) Antibody**
The CaMKII (Ab-286) antibody is a widely used tool in neuroscience and cardiovascular research, targeting the calcium/calmodulin-dependent protein kinase II (CaMKII) phosphorylated at threonine 286 (Thr286). CaMKII is a multifunctional serine/threonine kinase critical for synaptic plasticity, memory formation, and cardiac function. Its activation relies on calcium-calmodulin binding, triggering autophosphorylation at Thr286. which sustains kinase activity even after calcium levels decline. This autonomous activity underpins its role in long-term potentiation (LTP) and pathological conditions like arrhythmias or neurodegeneration.
The Ab-286 antibody specifically detects the autophosphorylated, active form of CaMKIIα, the predominant isoform in neurons. Researchers use it to study CaMKII activation dynamics in response to stimuli (e.g., synaptic activity, stress) or in disease models (e.g., Alzheimer’s, heart failure). It is validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence, providing insights into spatial and temporal kinase regulation. By distinguishing active CaMKII from its inactive state, Ab-286 helps elucidate mechanisms underlying learning, memory, and CaMKII-linked pathologies, making it indispensable for exploring cellular signaling pathways.
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