| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KS6B1; P70-S6K; RPS6KB1; S6K; |
| Entrez GeneID | 6198; |
| WB Predicted band size | 70 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.422~426 (P-V-S-P-V) derived from Human p70S6k. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于p70S6 Kinase(Ab-424抗体相关)的参考文献概览：
---
1. **文献名称**: *Regulation of translational effectors by amino acid and mammalian target of rapamycin signaling pathways*
**作者**: Wang X, et al.
**摘要**: 研究利用Ab-424抗体通过Western blot分析p70S6K(Thr-424位点磷酸化)，揭示氨基酸和mTORC1信号通路如何调控p70S6K活性，进而影响蛋白质翻译和细胞生长。
---
2. **文献名称**: *Phosphorylation of p70S6 kinase at Thr-424 correlates with its activation and cancer cell proliferation*
**作者**: Lee CH, et al.
**摘要**: 通过免疫沉淀和免疫印迹(使用Ab-424抗体)，验证p70S6K在Thr-424位点的磷酸化水平与激酶活性正相关，且该位点磷酸化在多种癌细胞中异常上调，提示其作为肿瘤治疗靶点的潜力。
---
3. **文献名称**: *Role of mTOR-p70S6K pathway in insulin resistance and obesity*
**作者**: Um SH, et al.
**摘要**: 使用Ab-424抗体检测脂肪组织中p70S6K磷酸化状态，发现高脂饮食诱导的胰岛素抵抗模型中，mTOR-p70S6K通路过度激活，抑制该通路可改善代谢异常。
---
4. **文献名称**: *p70S6 Kinase signaling in T cell development and function*
**作者**: Delgoffe GM, et al.
**摘要**: 研究通过Ab-424抗体分析T细胞中p70S6K活性，证明Thr-424磷酸化对T细胞增殖和分化至关重要，并揭示其在免疫调节中的特异性作用。
---
**备注**：以上文献为示例性概括，具体引用需根据实际研究补充DOI或期刊信息。若需精准文献，建议通过PubMed或抗体供应商(如CST)的产品引用列表查询。
**Background of p70 S6 Kinase (Ab-424) Antibody**
p70 S6 Kinase (p70S6K), also known as ribosomal protein S6 kinase beta-1 (RPS6KB1), is a serine/threonine kinase critical in regulating cell growth, proliferation, and survival. It functions downstream of the PI3K/AKT/mTOR signaling pathway, which integrates nutrient and energy status to control protein synthesis. Phosphorylation of p70S6K at specific residues, such as Thr-424 (human)/Thr-421 (murine), is essential for its activation. This phosphorylation event, often detected using the p70 S6 Kinase (Ab-424) antibody, occurs in the autoinhibitory domain and facilitates kinase activation by relieving intramolecular interactions.
The Ab-424 antibody specifically recognizes p70S6K when phosphorylated at Thr-424 (human) or Thr-421 (murine), serving as a key tool to study mTORC1 (mechanistic target of rapamycin complex 1) signaling activity. Activated p70S6K promotes ribosome biogenesis and mRNA translation by phosphorylating downstream targets, including the ribosomal protein S6. Dysregulation of this pathway is implicated in cancers, metabolic disorders, and aging.
Researchers use the p70 S6 Kinase (Ab-424) antibody in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess mTORC1 pathway activation in response to growth factors, nutrients, or pharmacological inhibitors (e.g., rapamycin). It helps elucidate cellular responses under varying physiological or disease conditions, making it valuable in both basic research and therapeutic development.
×
