| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADRB2; E2AK2; EIF2AK2; EIF2aK; PRKR |
| Entrez GeneID | 5610; |
| WB Predicted band size | 68kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Peptide sequence around aa.444~448 (K-R-T-R-S) derived from Human PKR. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及PKR抗体(可能包含Ab-446相关应用)的文献摘要，供参考：
1. **文献名称**: "Double-stranded RNA-dependent protein kinase activates transcription factor NF-κB by phosphorylating IκB"
**作者**: Kumar A, et al.
**摘要**: 研究利用PKR特异性抗体(包括Ab-446)进行Western blot分析，发现PKR通过磷酸化IκB激活NF-κB通路，揭示了其在炎症和免疫应答中的调控机制。
2. **文献名称**: "Role of PKR in the antiviral response against influenza virus"
**作者**: Garcia MA, et al.
**摘要**: 通过免疫沉淀(使用PKR抗体Ab-446)证实流感病毒激活PKR依赖性eIF2α磷酸化，抑制病毒蛋白合成，表明PKR是抗病毒天然免疫的关键介质。
3. **文献名称**: "PKR regulates proliferation and differentiation of human keratinocytes"
**作者**: Abraham N, et al.
**摘要**: 使用PKR抗体(Ab-446)进行免疫组化，发现PKR通过调控细胞周期蛋白抑制皮肤角质细胞过度增殖，为银屑病等疾病提供潜在治疗靶点。
注：若需具体文献链接或补充数据，建议通过抗体供应商(如Santa Cruz、Abcam等)的产品引用列表或PubMed高级检索(关键词：PKR antibody Ab-446)进一步筛选。部分文献可能因抗体编号差异需结合实验背景确认。
The PKR (Ab-446) antibody is a monoclonal antibody specifically designed to target the double-stranded RNA (dsRNA)-activated protein kinase (PKR), also known as EIF2AK2. PKR is a serine/threonine kinase that plays a critical role in innate immunity, particularly in antiviral defense and cellular stress responses. Upon binding to viral or endogenous dsRNA, PKR undergoes autophosphorylation, leading to its activation. Activated PKR phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF2α), which globally inhibits protein synthesis to limit viral replication. Beyond its antiviral functions, PKR is implicated in regulating apoptosis, inflammation, and signal transduction pathways linked to cancer, neurodegeneration, and metabolic disorders.
The Ab-446 antibody, often raised in rabbits, recognizes endogenous levels of total PKR protein across human, mouse, and rat samples. It is widely used in research applications such as Western blotting, immunoprecipitation, and immunofluorescence to investigate PKR expression, activation states, and interactions with other signaling molecules. Studies utilizing this antibody have contributed to elucidating PKR's role in stress granule formation, interferon signaling, and its crosstalk with pathways like NF-κB and mTOR. As dysregulation of PKR is associated with diseases such as Alzheimer’s, cancer, and viral infections, the Ab-446 antibody serves as a vital tool for exploring PKR's mechanistic contributions to pathophysiology and potential therapeutic targeting.
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