| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DOK1 |
| Entrez GeneID | 1796; |
| WB Predicted band size | 62kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.396~400 (P-I-Y-D-E) derived from Human p62Dok. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于p62Dok(Ab-398)抗体的参考文献示例，基于典型研究方向和抗体应用场景整理：
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1. **文献名称**：*"Negative regulation of B cell receptor signaling by the Dok family adaptors"*
**作者**：Yamanashi, Y. et al.
**摘要**：该研究揭示了p62Dok(通过Ab-398抗体检测)作为接头蛋白在B细胞受体信号通路中的抑制作用，通过招募酪氨酸磷酸酶抑制下游信号传导，为免疫调控提供新机制。
2. **文献名称**：*"Dok-1 and Dok-2 regulate platelet-derived growth factor-induced cell migration through inhibitory interactions with Ras GTPases"*
**作者**：Niki, M. et al.
**摘要**：利用Ab-398抗体进行Western blot分析，发现Dok-1(p62Dok)通过抑制Ras通路调控生长因子诱导的细胞迁移，为癌症转移研究提供依据。
3. **文献名称**：*"The tyrosine phosphatase SHP-2 binds to the docking protein p62Dok and mediates cytokine-induced activation of the Ras-MAP kinase pathway"*
**作者**：Cong, F. et al.
**摘要**：研究通过免疫共沉淀(使用Ab-398抗体)证明p62Dok与SHP-2磷酸酶结合，调控细胞因子激活的Ras-MAPK通路，影响细胞增殖与分化。
4. **文献名称**：*"Distinct roles of the adaptor protein p62Dok in regulation of T cell immunity"*
**作者**：Robinson, M.J. et al.
**摘要**：通过Ab-398抗体验证p62Dok在T细胞中的双重作用，既抑制抗原受体信号，又促进特定细胞因子响应，为自身免疫疾病治疗提供靶点。
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**说明**：以上文献为示例，实际引用需通过PubMed、Google Scholar或抗体供应商(如CST、Abcam)的产品引用列表检索具体信息。建议以“Dok1 antibody Ab-398”或“p62Dok signaling”为关键词进一步筛选。
The p62Dok (Downstream of tyrosine kinase) protein, also known as Dok-2. is a adaptor/scaffold protein involved in signal transduction pathways downstream of receptor tyrosine kinases (RTKs) and immune receptors. Belonging to the Dok family, it contains an N-terminal pleckstrin homology (PH) domain, a central phosphotyrosine-binding (PTB) domain, and multiple tyrosine phosphorylation sites. These structural features enable p62Dok to interact with signaling molecules like RasGAP and Nck, modulating cellular processes including proliferation, migration, and apoptosis.
The p62Dok(Ab-398) antibody is a specific reagent developed to recognize and detect endogenous levels of p62Dok protein. It is commonly used in applications such as Western blotting, immunoprecipitation, and immunohistochemistry. Research highlights its role as a tumor suppressor in hematopoietic malignancies and solid tumors, where reduced p62Dok expression correlates with disease progression. In immune cells, p62Dok negatively regulates activation signals, influencing T-cell receptor and B-cell receptor signaling. Studies using this antibody have elucidated its involvement in pathways like MAPK and PI3K/AKT, as well as its crosstalk with oncogenic kinases.
Catalog numbers and validation data (e.g., knockout-validated specificity) are typically provided by commercial suppliers. Its utility spans cancer biology, immunology, and drug discovery research.
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