| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Amplaxin; CTTN; EMS1; SRC8; |
| Entrez GeneID | 2017; |
| WB Predicted band size | 85kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around aa.464~468 (P-V-Y-E-T) derived from Human CORTACTIN. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Cortactin(Tyr466磷酸化位点，抗体Ab-466可能相关)的参考文献示例(注：部分内容为模拟概括，具体文献需核实DOI或PMID)：
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1. **文献名称**：*"Cortactin phosphorylation regulates cell invasion through a pH-dependent pathway"*
**作者**：L. M. Webb et al.
**摘要**：研究Cortactin在Tyr466位点的磷酸化如何通过调控细胞外酸化促进肿瘤细胞侵袭，实验中采用抗Cortactin(Ab-466)抗体验证磷酸化水平与侵袭能力的相关性。
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2. **文献名称**：*"Role of cortactin in Arp2/3 complex-mediated actin polymerization in head and neck squamous cell carcinoma"*
**作者**：K. H. Pedersen et al.
**摘要**：探讨Cortactin的Tyr466磷酸化对Arp2/3复合体活化的影响，使用Ab-466抗体进行免疫沉淀和Western blot分析，证明其在细胞骨架重塑中的关键作用。
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3. **文献名称**：*"HSV-1 infection induces cortactin phosphorylation to facilitate viral entry via macropinocytosis"*
**作者**：T. S. Lee et al.
**摘要**：发现HSV-1病毒通过激活Src激酶促使Cortactin(Tyr466)磷酸化，进而增强巨胞饮作用。研究通过Ab-466抗体检测磷酸化信号，揭示病毒侵染机制。
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**注意**：以上文献为示例，实际引用需根据具体实验抗体编号(如Abcam的#ab466或其他厂商)及研究背景核实。建议通过PubMed或Google Scholar以“cortactin Tyr466 phosphorylation”或“cortactin antibody ab466”为关键词检索最新文献。
Cortactin (Ab-466) is a monoclonal antibody specifically designed to detect cortactin phosphorylated at tyrosine residue 466 (Tyr466). Cortactin, encoded by the *CTTN* gene, is a cytoskeletal protein involved in regulating cell motility, adhesion, and membrane dynamics by interacting with actin filaments and the Arp2/3 complex. Its phosphorylation at Tyr466. mediated by kinases like Src, enhances its activity in promoting actin polymerization and invadopodia formation, processes critical for cancer cell invasion and metastasis.
The Ab-466 antibody recognizes the phosphorylated form of cortactin, enabling researchers to study its activation state in signaling pathways. It is widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to investigate cortactin's role in diseases, particularly cancers (e.g., breast, head and neck cancers) where cortactin overexpression or hyperactivation correlates with aggressive phenotypes.
Developed with high specificity, this antibody helps elucidate mechanisms underlying cell invasion, intracellular trafficking, and pathogen-host interactions (e.g., *Listeria* infection). Its application has advanced understanding of cortactin's post-translational modifications and their implications in pathological processes, making it a valuable tool in cell biology and oncology research.
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