Rabbit Polyclonal eIF4E(Ab-209) Antibody

The eIF4E (Ab-209) antibody is a specific immunological tool designed to detect eukaryotic translation initiation factor 4E (eIF4E), a critical protein involved in mRNA translation initiation. eIF4E binds to the 5' cap structure of mRNAs, facilitating their recruitment to the ribosome and regulating protein synthesis. Dysregulation of eIF4E is implicated in cancer, viral infections, and cellular stress responses, as its overexpression enhances translation of oncogenes and pro-growth mRNAs. The "Ab-209" designation typically refers to an antibody targeting a specific phosphorylation site or epitope within eIF4E. For example, phosphorylation at Ser209 (a common modification) modulates eIF4E’s activity and its interaction with other initiation factors. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunoprecipitation to study eIF4E expression, post-translational modifications, or its role in signaling pathways (e.g., mTOR or MAPK). Validated in specific experimental models, the antibody helps elucidate mechanisms of translational control in diseases, therapeutic resistance, or viral replication. Its specificity is often confirmed using knockout cells or competitive peptides. When using this antibody, expected band sizes (~25 kDa for unmodified eIF4E) and tissue-specific expression patterns should be considered.