| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EIF4EBP1; PHAS-1; |
| Entrez GeneID | 1978; |
| WB Predicted band size | 18kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.44~48 (S-T-T-P-G) derived from Human 4E-BP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于4E-BP1(Ab-46抗体)的3篇代表性文献信息，涵盖其功能研究和抗体应用场景：
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1. **文献名称**: "Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism"
**作者**: Gingras AC, et al.
**摘要**: 该研究通过免疫印迹(使用4E-BP1(Ab-46)抗体)揭示了mTORC1信号通路调控4E-BP1磷酸化的分子机制，发现其磷酸化过程依赖雷帕霉素敏感途径，并验证了Thr37/46位点磷酸化对eIF4E结合的抑制作用。
2. **文献名称**: "Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 in a rapamycin-sensitive manner"
**作者**: Hara K, et al.
**摘要**: 研究利用4E-BP1(Ab-46)抗体检测细胞在氨基酸充足/缺乏条件下的蛋白磷酸化状态，证实mTOR通过调控4E-BP1磷酸化水平影响翻译起始复合物的形成，从而参与细胞生长调控。
3. **文献名称**: "mTORC1-mediated cell proliferation, but not cell growth, controlled by the 4E-BPs"
**作者**: Dowling RJ, et al.
**摘要**: 通过4E-BP1(Ab-46)抗体的免疫沉淀实验，发现mTORC1依赖的4E-BP1磷酸化通过释放eIF4E促进特定mRNA的翻译，进而调控细胞周期进程而非细胞体积增长，为癌症靶向治疗提供理论依据。
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**注**：以上文献中使用的4E-BP1(Ab-46)抗体通常指向Cell Signaling Technology公司产品(货号#9452)，特异性识别Thr37/46磷酸化位点，广泛用于Western blot及信号通路研究。如需具体文献DOI或年份，可进一步补充数据库检索结果。
The 4E-BP1 (Ab-46) antibody is a widely used tool in molecular biology for studying the regulation of protein synthesis and cellular growth. 4E-BP1 (eIF4E-binding protein 1), also known as PHAS-I, is a key downstream effector of the mTORC1 signaling pathway. It binds to the eukaryotic translation initiation factor eIF4E, inhibiting cap-dependent mRNA translation by preventing the assembly of the eIF4F complex. Phosphorylation of 4E-BP1 at specific residues (e.g., Thr37/46) by mTORC1 disrupts this interaction, releasing eIF4E to promote translation initiation—a critical process in cell proliferation, survival, and stress responses.
The 4E-BP1 (Ab-46) antibody specifically recognizes total 4E-BP1 protein, regardless of its phosphorylation status, making it valuable for quantifying protein expression levels in various experimental models. It is commonly used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to investigate mTOR pathway activity, cellular metabolic states, and responses to stimuli like growth factors, nutrients, or stress. Dysregulation of 4E-BP1 phosphorylation is implicated in cancer, metabolic disorders, and aging, underscoring its relevance in both basic research and therapeutic target validation. The Ab-46 clone is favored for its reliability in detecting multiple isoforms of 4E-BP1 across species, including human, mouse, and rat samples.
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