Rabbit Polyclonal mTOR(Ab-2448) Antibody

The mTOR (Ab-2448) antibody is a widely used tool in molecular biology research to study the mechanistic target of rapamycin (mTOR), a serine/threonine kinase critical in regulating cell growth, metabolism, proliferation, and survival. This antibody specifically detects mTOR phosphorylated at the Serine 2448 (Ser2448) residue, a key post-translational modification site. Phosphorylation at Ser2448 is often associated with mTOR activation, particularly in response to growth factors, nutrients, or insulin signaling. It is linked to the mTOR complex 1 (mTORC1), which integrates upstream signals from pathways like PI3K/AKT and AMPK. Researchers employ this antibody in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to assess mTOR activation status in diverse contexts, including cancer, metabolic disorders, and aging. Its specificity for the phosphorylated form allows differentiation between active and inactive mTOR states. Validation typically includes testing in cell lines with genetic mTOR modifications or under stimulation/inhibition conditions (e.g., rapamycin treatment). The antibody’s utility extends to both basic research and preclinical studies, aiding in elucidating mTOR-related signaling networks and therapeutic targeting. Proper experimental controls, such as using phosphorylation-blocking agents or knockout models, are recommended to confirm signal specificity.