Rabbit Polyclonal b-Catenin(Ab-33) Antibody

The b-Catenin (Ab-33) antibody is a monoclonal antibody specifically designed to detect β-catenin, a multifunctional protein encoded by the CTNNB1 gene. β-Catenin plays dual roles in cellular processes: as a structural component of adherens junctions, it interacts with E-cadherin to regulate cell-cell adhesion; as a key transcriptional co-activator in the Wnt signaling pathway, it translocates to the nucleus to mediate gene expression involved in cell proliferation, differentiation, and tissue homeostasis. Dysregulation of β-catenin is implicated in various cancers, particularly colorectal and hepatocellular carcinomas, due to mutations in Wnt pathway components or CTNNB1 itself. The Ab-33 clone recognizes an epitope near the Tyr-333 residue of human β-catenin, enabling the detection of both wild-type and phosphorylated forms across applications such as Western blotting, immunofluorescence, and immunohistochemistry. Its specificity for β-catenin over other catenin family members (e.g., γ-catenin/plakoglobin) makes it valuable for studying Wnt signaling activation, epithelial-mesenchymal transition (EMT), and tumor progression. Researchers frequently use this antibody to assess β-catenin subcellular localization—nuclear accumulation being a hallmark of Wnt pathway activation—and to investigate therapeutic targets in cancer and developmental biology. Proper experimental controls, including cell lines with known β-catenin expression or knockout models, are recommended to validate staining specificity.