Rabbit Polyclonal Pyk2(Ab-402) Antibody

The Pyk2 (Ab-402) antibody is a widely used tool in biomedical research to study the proline-rich tyrosine kinase 2 (Pyk2), also known as protein tyrosine kinase 2 beta (PTK2B). Pyk2 is a non-receptor tyrosine kinase belonging to the focal adhesion kinase (FAK) family, which plays critical roles in cellular signaling pathways regulating cell adhesion, migration, proliferation, and survival. It is activated by various stimuli, including calcium influx, stress signals, and G-protein-coupled receptor activation, leading to autophosphorylation at key tyrosine residues (e.g., Tyr402) and downstream signaling via MAPK, PI3K/Akt, and other pathways. The Ab-402 antibody specifically recognizes the phosphorylated form of Pyk2 at Tyr402. a critical autophosphorylation site essential for its kinase activity and interaction with Src-family kinases. Researchers employ this antibody in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate Pyk2 activation in diverse biological contexts, including cancer metastasis, neuronal plasticity, bone remodeling, and immune responses. Its specificity and reliability have made it a valuable reagent for elucidating Pyk2's role in diseases such as osteoporosis, neurodegenerative disorders, and tumor progression. Proper validation using knockout controls is recommended to ensure target specificity, as cross-reactivity with related kinases like FAK has been reported in some cases.