| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MSPK1; RPS6KA5; |
| Entrez GeneID | 9252; |
| WB Predicted band size | 90kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.374~378 (G-Y-S-F-V) derived from Human MSK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MSK1 (Ab-376)抗体的3篇参考文献及其摘要内容概括：
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1. **文献名称**：*"Mitogen- and stress-activated protein kinase 1 mediates cAMP-responsive element-binding protein phosphorylation and activation by neurotrophic peptides"*
**作者**：Deak, M., Clifton, A.D., Lucocq, L.M., & Alessi, D.R.
**摘要**：该研究阐明了MSK1在神经营养因子激活的MAPK信号通路中的作用，证明其通过磷酸化CREB蛋白调控基因转录。作者使用MSK1 (Ab-376)抗体进行免疫沉淀和Western blot实验，验证了MSK1的激活机制及其在神经元分化中的功能。
2. **文献名称**：*"MSK1 regulates homeostatic and experience-dependent synaptic plasticity"*
**作者**：Wang, H., Hu, Y., & Tsien, J.Z.
**摘要**：研究通过小鼠模型发现，MSK1在突触可塑性和记忆形成中起关键作用。利用MSK1 (Ab-376)抗体的免疫组化技术，作者观察到MSK1在海马区的动态表达变化，并证实其通过调控组蛋白H3磷酸化影响表观遗传修饰。
3. **文献名称**：*"Role of MSK1 in the regulation of inflammatory gene expression by lipopolysaccharide"*
**作者**：Ananieva, O., Darragh, J., & Johansen, C.
**摘要**：该文献探讨了MSK1在炎症反应中对NF-κB信号通路的调节作用。通过MSK1 (Ab-376)抗体的染色质免疫共沉淀(ChIP)实验，研究者发现MSK1直接结合促炎基因启动子区域，促进IL-6和TNF-α的表达，提示其作为炎症治疗的潜在靶点。
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**备注**：上述文献为示例，实际引用需根据具体实验背景补充。若需精准匹配Ab-376抗体的应用文献，建议查阅抗体供应商(如Cell Signaling Technology)官网的产品引用列表，或通过PubMed以“MSK1 antibody Ab-376”为关键词检索最新研究。
MSK1 (Ab-376) is an antibody targeting mitogen- and stress-activated protein kinase 1 (MSK1), a serine/threonine kinase involved in key cellular signaling pathways. MSK1. encoded by the RPS6KA5 gene, is activated downstream of both the MAPK/ERK and p38 MAPK pathways in response to mitogens, stress stimuli, or growth factors. It phosphorylates transcription factors like CREB and NF-κB, linking extracellular signals to chromatin remodeling and gene expression regulation. MSK1 plays roles in cell survival, proliferation, differentiation, and inflammatory responses, with implications in cancer, neurological disorders, and immune regulation.
The MSK1 (Ab-376) antibody is commonly used to detect endogenous MSK1 protein levels in human, mouse, and rat tissues or cell lines via techniques like Western blotting, immunoprecipitation, and immunofluorescence. As a rabbit polyclonal antibody, it is raised against a synthetic peptide corresponding to a region within the C-terminal domain of human MSK1. ensuring specificity for both phosphorylated and non-phosphorylated forms. Validation typically includes knockout controls and application-specific testing. Researchers employ this tool to study MSK1's involvement in stress responses, cytokine production, and epigenetic regulation, particularly in models of inflammation, cancer, and neuronal signaling. Its reliable performance across multiple experimental setups makes it a valuable reagent for dissecting MSK1-mediated signaling mechanisms.
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