Rabbit Polyclonal Ephrin-B2(Ab-330) Antibody

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     Gel: 8% SDS-PAGELysate: 40 ugLane: A549 cell lysatePrimary antibody: Ephrin-B2(Ab-330) Antibody at dilution 1/800Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilutionExposure time: 10 seconds    

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     All  Lanes: Ephrin-B2(Ab-330) Antibody at 1/1000 dilution.    

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     Immunofluorescent analysis of formalin-fixed, paraffin-embedded hela cells with 21196-lot 7326 at 1/75 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.    

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     Immunofluorescent analysis of formalin-fixed, paraffin-embedded C6 cells with 21196-lot 7326 at 1/75 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.    

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     21196-lot 7326 at 1/200 staining human renal clear cell carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.    

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     21196-lot 7326 at 1/200 staining human meningeal carcinomatosis(MC) tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary    

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     21196-lot 7326 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary    

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     21196-lot 7326 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.    

Ephrin-B2 (Ab-330) is a monoclonal antibody specifically designed to detect Ephrin-B2. a key transmembrane ligand in the Eph/ephrin signaling pathway. Ephrin-B2 belongs to the B-class ephrins, which bind to Eph receptor tyrosine kinases to regulate bidirectional cell-cell communication. This ligand plays critical roles in developmental processes, including angiogenesis, tissue patterning, and neural connectivity, by mediating cell adhesion, migration, and boundary formation. It is particularly essential in vascular system development, where it guides arterial-venous differentiation and blood vessel remodeling.

The Ab-330 antibody targets a specific epitope within the intracellular domain of human Ephrin-B2. often recognizing phosphorylated tyrosine residues (e.g., Tyr-330) to study post-translational modifications linked to signaling activity. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to evaluate Ephrin-B2 expression and localization in tissues or cultured cells. Research applications focus on cancer biology, neurodevelopment, and cardiovascular diseases, as dysregulated Ephrin-B2 signaling is implicated in tumor angiogenesis, metastasis, and vascular malformations. Produced in host species like rabbits or mice, this antibody provides high specificity and reliability, making it a valuable tool for elucidating Ephrin-B2’s functional mechanisms in both physiological and pathological contexts.