| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AMPK, AMPKa1 |
| Entrez GeneID | 5562; |
| WB Predicted band size | 63kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa. 181~185/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"AMP-activated protein kinase: nature's energy sensor"**
*Hardie DG et al.*
该综述阐述了AMPKα1/α2亚基在细胞能量稳态中的核心作用，利用特异性抗体(包括Ab-183/172)验证其磷酸化激活机制，揭示其在代谢疾病中的调控靶点。
2. **"AMPKα1-deficient mice exhibit abnormal glucose tolerance"**
*Jørgensen SB et al.*
通过AMPKα1/α2 (Ab-183/172)抗体检测组织特异性基因敲除模型，发现AMPKα1缺失导致小鼠糖代谢紊乱，强调该亚基在肝脏胰岛素信号中的关键作用。
3. **"Pharmacological activation of AMPK improves atherosclerotic plaque stability"**
*Li Y et al.*
研究使用Ab-183/172抗体证明AMPKα1/α2磷酸化水平与动脉粥样硬化斑块稳定性相关，激活AMPK可抑制炎症并促进斑块修复。
4. **"AMPKα2 mediates mitochondrial fission in cardiac ischemia-reperfusion injury"**
*Wang S et al.*
利用该抗体发现心肌缺血再灌注中AMPKα2亚基通过调控线粒体分裂蛋白Drp1加重损伤，靶向AMPKα2或为治疗策略。
注：以上文献名为示例，实际引用需根据具体研究匹配。Ab-183/172抗体常用于检测AMPKα1/α2在Thr172/183位点的磷酸化状态，广泛用于代谢、癌症及心血管疾病机制研究。
The AMP-activated protein kinase (AMPK) is a crucial cellular energy sensor that regulates metabolic homeostasis by responding to fluctuations in energy status. It exists as a heterotrimeric complex comprising a catalytic α subunit (α1 or α2) and regulatory β and γ subunits. AMPKα1 (encoded by PRKAA1) and AMPKα2 (PRKAA2) share structural homology but exhibit tissue-specific expression patterns, with α1 being ubiquitously expressed and α2 predominant in skeletal muscle, liver, and heart. Activation occurs through phosphorylation at Thr172 (α subunit) by upstream kinases like LKB1 or CaMKKβ, particularly under energy-deprived conditions.
The AMPKα1/AMPKα2 (Ab-183/172) antibody specifically detects endogenous levels of AMPKα1 and α2 subunits when phosphorylated at critical residues. The "Ab-183" designation typically refers to recognition of a conserved phospho-epitope (often at Ser173/Ser177 in α1/α2. respectively), while "Ab-172" indicates detection of the essential Thr172 phosphorylation required for kinase activation. This dual-specificity antibody enables researchers to study AMPK activation status across various biological contexts without distinguishing between α isoforms.
Widely used in techniques like Western blotting and immunohistochemistry, this antibody facilitates investigations into metabolic disorders, cancer, and aging. It helps elucidate AMPK's role in glucose/lipid metabolism, autophagy, and cellular stress responses. Proper controls, including knockout validation and phosphatase treatment, are recommended to confirm signal specificity due to potential cross-reactivity with other kinases sharing similar phosphorylation motifs.
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