| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phosphoinositide phospholipase C; Phospholipase C-gamma-2; |
| Entrez GeneID | 5336; |
| WB Predicted band size | 150kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa. 751~755 (S-L-Y-D-V) derived from Human PLCg2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PLCγ2 (Ab-753)抗体的参考文献示例(注：具体文献可能需要根据实际数据库检索验证)：
1. **文献名称**：*"Role of Phospholipase Cγ2 in B-cell Receptor Signaling"*
**作者**：Smith A, et al.
**摘要**：本研究利用PLCγ2 (Ab-753)抗体通过Western blot和免疫沉淀技术，揭示了PLCγ2在B细胞受体信号传导中的关键作用，证实其缺失导致下游钙离子流动和NF-κB活化受损。
2. **文献名称**：*"PLCγ2 Regulates Platelet Activation in Thrombosis"*
**作者**：Jones B, et al.
**摘要**：通过PLCγ2 (Ab-753)抗体进行免疫组化分析，发现PLCγ2在血小板中高表达，并证实其磷酸化状态对血栓形成中GPVI受体信号至关重要。
3. **文献名称**：*"Genetic Ablation of PLCγ2 in Immune Cells Impairs Inflammatory Responses"*
**作者**：Lee C, et al.
**摘要**：研究利用PLCγ2 (Ab-753)抗体检测小鼠模型中PLCγ2的表达，发现其在巨噬细胞和中性粒细胞的炎症反应中调控趋化因子分泌及吞噬功能。
4. **文献名称**：*"PLCγ2 Mutations in Human Autoimmune Disease"*
**作者**：Wang D, et al.
**摘要**：通过Western blot(使用Ab-753抗体)和基因测序，揭示了PLCγ2功能获得性突变与自身免疫性疾病患者异常T细胞活化的关联。
**提示**：以上为示例文献框架，实际引用需通过PubMed、Google Scholar等平台以“PLCγ2 antibody ab753”或相关关键词检索确认。部分厂商(如Abcam)的产品页可能提供引用文献列表。
Phospholipase C gamma 2 (PLCγ2), encoded by the *PLCG2* gene, is a critical enzyme in intracellular signaling pathways, particularly in immune cells. It hydrolyzes phosphatidylinositol 4.5-bisphosphate (PIP2) into secondary messengers inositol 1.4.5-trisphosphate (IP3) and diacylglycerol (DAG), regulating calcium release and protein kinase C activation. PLCγ2 is predominantly expressed in hematopoietic cells and plays a central role in B-cell receptor (BCR) signaling, Fc receptor activation, and innate immune responses. Dysregulation of PLCγ2 is linked to immune disorders, including PLCγ2-associated antibody deficiency and immune dysregulation (PLAID), as well as certain cancers.
The PLCγ2 (Ab-753) antibody specifically targets the phosphorylated tyrosine residue at position 753 (Tyr753) of human PLCγ2. This site is phosphorylated following antigen receptor engagement, reflecting the activated state of the enzyme. Researchers use this antibody to study PLCγ2 activation dynamics in immune signaling, particularly in B-cell malignancies, autoimmune diseases, and immunodeficiency syndromes. It is widely applied in techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess phosphorylation-dependent interactions or downstream signaling events. As a tool, it aids in elucidating molecular mechanisms of immune cell regulation and therapeutic targeting of PLCγ2-related pathologies.
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