Rabbit Polyclonal DNAPKcs(Ab-2609) Antibody

The DNA-dependent protein kinase catalytic subunit (DNA-PKcs), encoded by the PRKDC gene, is a critical serine/threonine kinase involved in the non-homologous end joining (NHEJ) pathway, the primary DNA repair mechanism for double-strand breaks (DSBs) in mammalian cells. DNA-PKcs forms a complex with Ku70/Ku80 heterodimers at DSB sites, enabling recruitment of repair factors and facilitating genomic stability. Dysregulation of DNA-PKcs is linked to radiation sensitivity, immune deficiencies, and cancer progression. The DNA-PKcs (Ab-2609) antibody is a widely used tool for detecting endogenous levels of DNA-PKcs in human and mouse models. It specifically recognizes the protein’s C-terminal region and is validated for applications including Western blotting, immunoprecipitation, and immunofluorescence. Researchers utilize this antibody to study DNA repair mechanisms, evaluate therapeutic responses to radiation or chemotherapy, and explore CRISPR-Cas9 gene-editing efficiency linked to NHEJ activity. Its specificity is often confirmed through siRNA knockdown or DNA-PKcs-deficient cell lines. As a rabbit polyclonal antibody, Ab-2609 offers high affinity and reproducibility, making it a staple in studies investigating genomic instability, cancer biology, and targeted therapy development.