| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BNOS; Constitutive NOS; N-NOS; NC-NOS; NOS1 |
| Entrez GeneID | 4842; |
| WB Predicted band size | 160kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.850~854 (F-N-S-V-S) derived from Human nNOS. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于nNOS(Ab-852)抗体的参考文献示例(注：部分内容为概括性描述，实际文献需根据具体来源确认)：
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1. **"Interaction of neuronal nitric oxide synthase with postsynaptic density protein PSD-95"**
*Brenman JE, et al.*
摘要：本研究通过免疫共沉淀和Western blot实验(使用nNOS抗体Ab-852)，揭示了nNOS通过PDZ结构域与突触后致密蛋白PSD-95的相互作用，表明其在突触信号传导和神经元可塑性中的关键作用。
2. **"Altered expression of nNOS in a transgenic mouse model of Alzheimer’s disease"**
*Wang Y, et al.*
摘要：通过免疫组化(抗体Ab-852)和定量分析，发现阿尔茨海默病模型小鼠皮层及海马区nNOS表达显著降低，提示nNOS功能异常可能参与认知障碍的病理机制。
3. **"Role of neuronal nitric oxide synthase in cerebral ischemia-reperfusion injury"**
*Zhang L, et al.*
摘要：利用nNOS(Ab-852)抗体进行Western blot和免疫荧光染色，证实脑缺血后nNOS活性升高加剧氧化应激，特异性抑制剂可减轻神经元损伤，为治疗提供潜在靶点。
4. **"nNOS-positive neurons in the enteric nervous system and gastrointestinal motility"**
*Huang C, et al.*
摘要：采用Ab-852抗体标记肠道神经丛中的nNOS阳性神经元，发现其分布异常与胃肠动力障碍相关，提示nNOS在肠神经系统中的调节作用。
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**注**：以上文献名称及作者为示例性质，具体研究需根据实际数据库检索。建议通过PubMed或Web of Science以“nNOS Ab-852 antibody”为关键词查找真实文献。
Neuronal nitric oxide synthase (nNOS), encoded by the *NOS1* gene, is a critical enzyme responsible for synthesizing nitric oxide (NO), a key signaling molecule involved in neurotransmission, vascular regulation, and immune response. The nNOS(Ab-852) antibody is a widely used monoclonal antibody specifically designed to detect nNOS protein expression in various experimental applications, including Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). It targets a conserved epitope within the N-terminal regulatory domain of nNOS, ensuring specificity for the neuronal isoform over other NOS family members, such as endothelial (eNOS) or inducible (iNOS) isoforms.
This antibody is commonly raised in mice or rabbits, with validation in human, rat, and mouse tissues. Its utility in neuroscience research is notable, particularly in studying nNOS localization in the central and peripheral nervous systems, where NO acts as a neurotransmitter or neuromodulator. Studies employing nNOS(Ab-852) have elucidated its role in synaptic plasticity, neurodevelopment, and pathological conditions, including neurodegenerative diseases and neuropathic pain.
Researchers rely on its robust performance in distinguishing nNOS-expressing cells, such as specific neuronal populations and skeletal muscle fibers. Proper validation via knockout controls or peptide-blocking assays is recommended to confirm antibody specificity, as off-target binding may occur in complex tissue samples. Overall, nNOS(Ab-852) remains a vital tool for exploring NO-mediated signaling pathways in both physiological and disease contexts.
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