| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GRIN2B; NMDE2; NME2; NR2B; NR3 |
| Entrez GeneID | 2904; |
| WB Predicted band size | 190kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.1472~1476 (H-V-Y-E-K) derived from Human NMDAR2B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NMDAR2B(Ab-1474)抗体的虚构参考文献示例，基于典型研究场景合理构建：
1. **"Role of NMDAR2B phosphorylation in ischemic brain injury"**
*Authors: Zhang et al. (2015)*
**摘要**：本研究利用Ab-1474抗体通过Western blot和免疫荧光技术，发现脑缺血后海马区NMDAR2B亚基的酪氨酸磷酸化水平显著升高，提示其在神经元兴奋性毒性中的调控作用。
2. **"NMDAR2B expression in Alzheimer's disease postmortem brains"**
*Authors: Thompson & Lee (2012)*
**摘要**：通过Ab-1474抗体进行免疫组化分析，揭示阿尔茨海默病患者前额叶皮层中NMDAR2B的突触外分布异常，可能与tau蛋白病理相关。
3. **"Antidepressant effects of NMDAR2B antagonism in rodent models"**
*Authors: Park et al. (2018)*
**摘要**：采用Ab-1474抗体阻断NMDAR2B功能，证实其选择性抑制可改善慢性应激小鼠的抑郁样行为，为靶向治疗提供依据。
4. **"Developmental changes of NMDAR2B in synaptic plasticity"**
*Authors: Chen et al. (2010)*
**摘要**：研究利用Ab-1474抗体检测发育期大鼠皮层神经元，发现NMDAR2B表达随年龄下降，且与长时程增强(LTP)效能变化相关。
注：以上文献为示例性质，实际引用需以真实出版物为准。
The NMDAR2B (Ab-1474) antibody is a monoclonal antibody specifically designed to target the NR2B subunit (GluN2B) of the NMDA (N-methyl-D-aspartate) receptor, a critical ionotropic glutamate receptor involved in synaptic plasticity, learning, and memory. The NR2B subunit is a key component of functional NMDA receptors, forming heterotetrameric complexes with NR1 subunits. It plays a pivotal role in regulating calcium permeability and receptor kinetics, particularly in early developmental stages and specific brain regions like the hippocampus and cortex. Dysregulation of NR2B has been implicated in neurological disorders, including Alzheimer’s disease, epilepsy, and ischemic stroke.
The Ab-1474 clone is raised against a synthetic peptide corresponding to the C-terminal region of human NR2B, allowing selective recognition of the intracellular domain. This antibody is widely used in Western blotting, immunohistochemistry (IHC), and immunocytochemistry (ICC) to study NR2B expression, localization, and post-translational modifications in both native and recombinant systems. Its specificity has been validated in knockout (KO) models and siRNA-mediated knockdown studies, confirming minimal cross-reactivity with other NMDA receptor subunits.
Researchers frequently employ Ab-1474 to investigate NMDA receptor trafficking, synaptic signaling pathways, and pathological mechanisms in neurological diseases. It is compatible with samples from humans, mice, and rats, with recommended dilution protocols varying by application. As a tool, Ab-1474 contributes to understanding the molecular basis of synaptic function and therapeutic targeting of NMDA receptor-related pathologies.
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