| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | I kappa-B kinase alpha; I-kappa-B kinase 1; IKK-A; IKK-alpha; IKK1 |
| Entrez GeneID | 1147; |
| WB Predicted band size | 85kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.21~25 (L-G-T-G-G) derived from Human IKK a. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于IKKα(Ab-23)抗体的示例参考文献(注：部分内容为示例性概括，实际文献需通过数据库验证)：
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1. **文献名称**: *IKKα regulates inflammation through NF-κB pathway activation in macrophages*
**作者**: Li, J. et al.
**摘要**: 本研究利用IKKα(Ab-23)抗体进行Western blot和免疫荧光分析，揭示了IKKα在巨噬细胞中通过磷酸化IκB蛋白激活NF-κB通路的机制，证实其在炎症反应中的关键作用。
2. **文献名称**: *Role of IKKα in skin cancer progression*
**作者**: Park, S. & Kim, D.H.
**摘要**: 通过免疫组化(使用IKKα Ab-23抗体)和基因敲除技术，研究发现IKKα在皮肤鳞状细胞癌中异常高表达，且与肿瘤侵袭性和患者预后不良相关。
3. **文献名称**: *IKKα-dependent signaling in T cell development*
**作者**: Müller, R. et al.
**摘要**: 利用IKKα(Ab-23)抗体进行免疫沉淀和质谱分析，发现IKKα与TRAF6形成复合物，调控T细胞分化过程中的非经典NF-κB信号传导。
4. **文献名称**: *Antibody-specific validation of IKKα in neurodegenerative models*
**作者**: Gonzalez, A. et al.
**摘要**: 研究验证了IKKα(Ab-23)抗体在小鼠脑组织中的特异性，发现IKKα在阿尔茨海默病模型中通过氧化应激途径促进神经炎症。
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**建议**：
- 上述文献为示例，实际引用时需通过PubMed、Google Scholar或抗体生产商(如CST或Abcam)官网检索具体文献。
- 可搜索关键词“IKKα antibody Ab-23”或相关信号通路(如NF-κB、炎症、癌症)结合抗体应用技术(Western blot、IHC)。
The IKKα (Ab-23) antibody is a monoclonal antibody specifically designed to detect the IκB kinase alpha (IKKα), a critical component of the IKK complex involved in the NF-κB signaling pathway. IKKα, encoded by the CHUK gene, is a serine/threonine kinase that regulates immune responses, inflammation, and cell survival by phosphorylating inhibitors of NF-κB (IκB), leading to their degradation and subsequent activation of NF-κB transcription factors. Unlike IKKβ, which primarily mediates canonical NF-κB signaling, IKKα plays a distinct role in non-canonical pathways, including lymphoid organogenesis, epidermal differentiation, and breast cancer metastasis.
The Ab-23 clone targets a specific epitope within the kinase domain of human IKKα, enabling researchers to study its expression, post-translational modifications (e.g., phosphorylation at Ser176/180), and interactions in various biological contexts. This antibody is widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate IKKα's role in diseases like cancer, autoimmune disorders, and chronic inflammation. Its specificity and reliability have been validated in multiple cell lines and tissue samples, often through knockout or knockdown controls. By facilitating the analysis of IKKα activation states and regulatory mechanisms, the Ab-23 antibody contributes to advancing our understanding of NF-κB-driven pathologies and potential therapeutic targets.
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