| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FLJ16691; PXN; |
| Entrez GeneID | 5829; |
| WB Predicted band size | 68kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa. 116~120 (H-V-Y-S-F) derived from Human Paxillin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Paxillin(Tyr-118磷酸化位点，可能对应抗体Ab-118)的模拟参考文献示例，内容基于相关研究领域常见主题，供参考：
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1. **文献名称**：*"FAK-mediated phosphorylation of paxillin at Tyr-118 regulates cell migration and adhesion dynamics"*
**作者**：Turner CE, Brown MC, Perrotta JA
**摘要**：本研究利用特异性识别Paxillin Tyr-118磷酸化位点的抗体(Ab-118)，揭示了FAK激酶在黏着斑信号中的关键作用。通过免疫荧光和Western blot分析，发现Y118位点的磷酸化促进Paxillin与整合素β1的相互作用，调控细胞迁移和黏着斑重塑。
2. **文献名称**：*"Role of paxillin tyrosine phosphorylation in breast cancer metastasis"*
**作者**：Zhao J, Guan JL
**摘要**：作者使用Paxillin (Ab-118)抗体检测侵袭性乳腺癌细胞中Tyr-118的磷酸化水平，发现其高表达与转移能力正相关。实验表明，抑制该位点磷酸化可降低癌细胞侵袭，提示其作为潜在治疗靶点。
3. **文献名称**：*"Integrin signaling through paxillin: Structural and functional analysis of phosphorylation sites"*
**作者**：Deakin NO, Turner CE
**摘要**：通过免疫沉淀(IP)和质谱技术，结合Ab-118抗体验证Paxillin Tyr-118磷酸化在整合素激活后的动力学变化。研究发现该位点磷酸化促进Rac1通路激活，影响细胞铺展和运动。
4. **文献名称**：*"Mechanical tension regulates paxillin phosphorylation and focal adhesion assembly"*
**作者**：Hirata H, Tatsumi H, Sokabe M
**摘要**：利用Ab-118抗体及力学刺激实验，证明细胞外基质刚度通过增强Paxillin Tyr-118磷酸化调控黏着斑成熟，为机械信号转导机制提供了分子证据。
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**注**：以上文献为模拟示例，实际引用需以真实发表的论文为准。建议通过PubMed或Google Scholar以“paxillin tyrosine 118 phosphorylation”为关键词检索相关研究。
Paxillin (Ab-118) antibody is a specific immunological tool designed to detect the phosphorylated form of paxillin at tyrosine residue 118 (Tyr118). Paxillin is a multifunctional adaptor protein localized at focal adhesions, where it plays a critical role in mediating cell-matrix adhesion, cytoskeletal organization, and intracellular signaling. It contains multiple protein-binding domains, including LD motifs and LIM domains, enabling interactions with kinases (e.g., FAK, Src), structural proteins (e.g., vinculin), and other signaling molecules. Phosphorylation of Tyr118 is a key regulatory event triggered by integrin engagement or growth factor stimulation, facilitating downstream signaling pathways involved in cell migration, proliferation, and survival.
The Paxillin (Ab-118) antibody is widely used in studies exploring focal adhesion dynamics, cell motility, and cancer metastasis. Its specificity for phosphorylated Tyr118 makes it valuable for assessing activation status of paxillin in response to extracellular cues. Common applications include Western blotting, immunofluorescence, and immunoprecipitation in cell lines or tissue samples. Researchers employ this antibody to investigate mechanisms underlying pathological processes such as tumor invasion, wound healing, and fibrosis. Validation typically involves confirming signal reduction via phosphatase treatment or site-directed mutagenesis. Proper controls are essential to ensure specificity, given the cross-reactivity risks with other phosphotyrosine epitopes.
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