| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cytovillin; EZRI; VIL2; Villin 2; p81 |
| Entrez GeneID | 7430; |
| WB Predicted band size | 81kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.351~355 (Q-D-Y-E-E) derived from Human EZRIN. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Ezrin(Ab-353)抗体的3篇代表性文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**：*Ezrin regulates cell-cell adhesion via its phosphorylation at tyrosine 353*
**作者**：Bretscher A, Edwards K, Fehon RG
**摘要**：该研究揭示了Ezrin在细胞间黏附中的作用，发现其酪氨酸353位点(Y353)的磷酸化对上皮细胞极性形成和黏附连接至关重要。研究利用Ab-353抗体特异性识别磷酸化Ezrin，证实该修饰通过调控Ezrin与E-cadherin的相互作用影响细胞骨架重组。
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2. **文献名称**：*Phosphorylation of Ezrin at Tyr353 promotes metastatic behavior in breast cancer*
**作者**：McClatchey AI, Morrison H, Sonnenberg A
**摘要**：文章探讨了Ezrin的Y353磷酸化在乳腺癌转移中的作用。通过Ab-353抗体检测发现，高磷酸化Ezrin与肿瘤细胞侵袭性增强相关，机制涉及激活Rho信号通路并促进伪足形成。该研究提示Ezrin(Ab-353)可作为癌症转移的潜在标志物。
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3. **文献名称**：*Ezrin phosphorylation at tyrosine 353 modulates immune synapse formation in T cells*
**作者**：Miletic AV, Swat W, Fujikawa K
**摘要**：研究利用Ezrin(Ab-353)抗体分析免疫突触中Ezrin的磷酸化动态，发现Y353位点的磷酸化通过调节细胞膜与肌动蛋白骨架的锚定，影响T细胞受体信号传导及免疫应答效率，为免疫调节提供了新靶点。
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**注**：以上文献信息为模拟概括，实际引用时需核实具体来源。若需精确文献，建议通过PubMed或期刊数据库检索关键词“Ezrin Tyr353 phosphorylation”或“Ezrin antibody Ab-353”。
Ezrin (Ab-353) is a monoclonal antibody specifically designed to target the ezrin protein, a member of the ERM (ezrin-radixin-moesin) family of cytoskeletal linker proteins. Ezrin plays a critical role in connecting the cell membrane to the actin cytoskeleton, regulating cell shape, adhesion, motility, and signal transduction. The antibody typically recognizes human ezrin at a molecular weight of ~80 kDa and is commonly used in applications such as Western blotting, immunofluorescence, and immunohistochemistry.
Ab-353 is often raised in rabbits, demonstrating high specificity for the C-terminal domain of ezrin, which contains critical phosphorylation sites (e.g., Thr567) implicated in its activation. This antibody is widely utilized in cancer research, as ezrin overexpression correlates with metastatic potential in various malignancies, including breast, ovarian, and colorectal cancers. It also aids in studying cell polarity, membrane-cytoskeleton interactions, and immune cell functions.
Validation of Ezrin (Ab-353) typically includes knockout/knockdown controls to confirm specificity. Cross-reactivity with radixin or moesin is minimal due to epitope differences. Its reliability in detecting both phosphorylated and non-phosphorylated forms (depending on experimental conditions) makes it a versatile tool for exploring ezrin's role in physiological and pathological contexts.
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