Rabbit Polyclonal p53(Ab-15) Antibody

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     Western blot analysis of lysates from 1)p53 knockout A431 cell , 2)A431 cells, (Green) primary antibody was diluted at 1:1000, 4°Cover night, Dylight 800 secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Actin β Monoclonal Antibody(5B7) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1hour.    

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     Immunofluorescence analysis of human-liver tissue. 1,p53 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B    

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     Immunofluorescence analysis of human-lung tissue. 1,p53 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B    

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     Immunofluorescence analysis of mouse-liver tissue. 1,p53 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B    

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     Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,p53 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.    

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     Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,p53 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.    

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     Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,p53 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.    

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     Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,p53 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.    

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     Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,p53 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.    

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     Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,p53 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.    

The p53(Ab-15) antibody is a monoclonal antibody specifically designed to detect the human tumor suppressor protein p53. encoded by the TP53 gene. p53 plays a critical role in regulating cell cycle arrest, DNA repair, apoptosis, and genomic stability in response to cellular stress. Mutations in TP53 are observed in ~50% of human cancers, often leading to loss of wild-type p53 function or accumulation of dysfunctional mutant p53 proteins, which contribute to tumor progression.

The Ab-15 clone recognizes an epitope within the N-terminal transactivation domain (amino acids 20-25) of both wild-type and mutant p53 isoforms. It is widely used in applications such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to study p53 expression, localization, and post-translational modifications in cancer research. Validated for use in formalin-fixed, paraffin-embedded (FFPE) tissues, this antibody helps assess p53 status in clinical samples, aiding in cancer diagnosis and prognosis. Its specificity and reliability make it a valuable tool for investigating p53's role in tumor suppression, therapeutic resistance, and cellular stress responses.