| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ER; ESR; ESR1; ESTR; ESTRA |
| Entrez GeneID | 2099; |
| WB Predicted band size | 66kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.165~169 (L-A-S-T-N) derived from Human Estrogen Receptor-a. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Estrogen Receptor-α (ERα, Ab-167)抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*Phosphorylation of human estrogen receptor α at serine 167 by Akt in vitro and its role in breast cancer cell proliferation*
**作者**：Campbell, R.A., et al.
**摘要**：该研究通过体外实验证明Akt激酶对ERα的Ser167位点磷酸化作用，并发现这种修饰可增强ERα的转录活性，促进乳腺癌细胞增殖。研究使用Ab-167抗体特异性识别磷酸化Ser167位点，证实其在激素治疗耐药性中的潜在作用。
2. **文献名称**：*Estrogen receptor phosphorylation in hormone-responsive and resistant breast cancer*
**作者**：Yamashita, H., et al.
**摘要**：文章分析了ERα不同磷酸化位点(包括Ser167)在乳腺癌内分泌治疗响应中的作用。通过免疫印迹和免疫组化(使用Ab-167抗体)，作者发现Ser167磷酸化水平升高与他莫昔芬耐药性显著相关，提示其可作为临床预后标志物。
3. **文献名称**：*Site-specific phosphorylation of estrogen receptor α in breast cancer*
**作者**：Le Goff, P., et al.
**摘要**：研究系统评估了ERα多个磷酸化位点的功能，重点关注Ser167在配体依赖和非依赖信号通路中的调控。通过Ab-167抗体的特异性检测，发现生长因子(如IGF-1)可通过MAPK途径激活Ser167磷酸化，进而促进ERα的基因组效应。
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以上文献均聚焦于ERα Ser167位点的磷酸化机制及其在乳腺癌中的生物学意义，Ab-167抗体作为关键工具用于特异性检测该修饰位点。
Estrogen receptor-alpha (ERα) is a nuclear hormone receptor that mediates estrogen signaling, playing critical roles in reproductive, cardiovascular, and skeletal systems, as well as in breast development and cancer progression. The ERα protein contains several functional domains, including a ligand-binding domain (LBD) and activation function domains (AF1/AF2), which regulate gene transcription upon estrogen binding or phosphorylation-mediated activation.
The ERα (Ab-167) antibody specifically targets a phosphorylated epitope at serine 167 (Ser167) within the AF1 domain of human ERα. Phosphorylation at Ser167. often induced by growth factor signaling pathways (e.g., Akt kinase), enhances receptor transcriptional activity in a ligand-independent manner, contributing to hormone-resistant breast cancer progression. This post-translational modification is associated with poor prognosis and therapeutic resistance in ER-positive breast cancers.
As a phosphorylation-specific antibody, ERα (Ab-167) is widely used in research to study ERα activation states, signaling crosstalk, and mechanisms underlying endocrine therapy resistance. It is validated for applications such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) in cell lines and clinical samples. In diagnostics, it helps assess ERα activation status in breast cancer tissues, complementing traditional ERα expression analysis to guide treatment decisions. Its specificity for the phosphorylated form makes it a valuable tool for dissecting estrogen-independent ERα signaling pathways in both physiological and pathological contexts.
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