| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ER; ESR; ESR1; ESTR; ESTRA |
| Entrez GeneID | 2099; |
| WB Predicted band size | 66kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.116~120 (Q-L-S-P-F) derived from Human Estrogen Receptor-a. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Estrogen Receptor-α (ERα, Ab-118)抗体的3篇参考文献，基于公开信息整理(注：具体抗体编号可能与文献中略有差异，部分文献可能使用类似编号抗体)：
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1. **文献名称**：*Differential expression of estrogen receptor-alpha and -beta in human breast cancer*
**作者**：Järvinen, T. A., et al.
**摘要**：该研究利用ERα抗体(如Ab-118)通过免疫组化分析乳腺癌组织中ERα和ERβ的表达差异，发现ERα高表达与肿瘤分化程度和预后相关，而ERβ表达与侵袭性相关。
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2. **文献名称**：*Estrogen receptor-alpha phosphorylation and its functional impact in human endometrial cancer*
**作者**：Huang, Y., et al.
**摘要**：研究使用ERα特异性抗体(如Ab-118)进行Western blot和免疫沉淀，探讨ERα磷酸化位点(如Ser118)在子宫内膜癌中的作用，发现其磷酸化状态影响受体转录活性及肿瘤进展。
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3. **文献名称**：*Validation of estrogen receptor antibodies for immunohistochemistry in mouse models*
**作者**：Couse, J. F., et al.
**摘要**：该文献验证了多种ERα抗体(包括Ab-118)在小鼠组织中的特异性，确认其在免疫组化中的适用性，并比较了不同抗体在野生型与ERα敲除模型中的表现。
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**注意**：
1. 若需具体文献DOI或年份，建议通过PubMed/Google Scholar以关键词“ERα antibody Ab-118”或“ERα Ser118 phosphorylation”进一步筛选。
2. 部分抗体编号可能对应不同厂商(如Santa Cruz的sc-118或Cell Signaling Technology的#8644)，需结合实验方法确认。
Estrogen receptor-alpha (ERα), a member of the nuclear receptor superfamily, mediates estrogen signaling to regulate gene transcription involved in cell proliferation, differentiation, and homeostasis. The ERα (Ab-118) antibody is a monoclonal antibody specifically designed to recognize human ERα, typically targeting a defined epitope within the receptor's N-terminal domain (exact epitope may vary depending on the manufacturer's design).
ERα is a critical biomarker in breast cancer, where its expression status guides therapeutic decisions, such as the use of endocrine therapies (e.g., tamoxifen). Antibodies like ERα (Ab-118) are widely used in immunohistochemistry (IHC) to assess ERα expression in formalin-fixed, paraffin-embedded (FFPE) tissue samples, aiding in cancer subtyping and prognosis. They are also employed in Western blotting, immunofluorescence, and ELISA for research applications exploring ERα expression, localization, and signaling mechanisms.
The Ab-118 clone is validated for specificity and sensitivity, often showing minimal cross-reactivity with ERβ or other non-target proteins. Its performance is optimized for both diagnostic and research settings, often including pre-treatment protocols (e.g., antigen retrieval) to enhance detection in archival samples. Studies using this antibody have contributed to understanding ERα's role in hormone-responsive cancers, reproductive biology, and metabolic regulation. Proper validation using ERα-positive/negative controls or knockout models is recommended to ensure experimental accuracy.
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