| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NBN |
| Entrez GeneID | 4683; |
| WB Predicted band size | 95kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.341~345 (S-L-S-Q-G) derived from Human p95/NBS1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于p95/NBS1(Ab-343)抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*NBS1 Phosphorylation Is Essential for DNA Damage Checkpoint Response*
**作者**：Kobayashi J. et al.
**摘要**：该研究通过免疫沉淀和Western blot分析，使用p95/NBS1(Ab-343)抗体证实了NBS1蛋白在DNA损伤后ATM依赖的磷酸化修饰，揭示了其在细胞周期检查点调控中的关键作用。
2. **文献名称**：*The Role of NBS1 in the Recruitment of MRE11 Complex to DNA Double-Strand Breaks*
**作者**：Desai-Mehta A. et al.
**摘要**：利用p95/NBS1(Ab-343)抗体进行免疫荧光染色，研究证明NBS1缺陷细胞中MRE11复合物无法有效定位至DNA损伤位点，强调了NBS1在DNA修复复合体组装中的桥梁功能。
3. **文献名称**：*Expression Analysis of NBS1 in Human Cancers Using a Specific Monoclonal Antibody*
**作者**：Yang M.H. et al.
**摘要**：通过p95/NBS1(Ab-343)抗体的免疫组化检测，发现NBS1在多种实体肿瘤中表达异常，提示其可能作为癌症预后标志物或治疗靶点。
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这些文献均以p95/NBS1(Ab-343)抗体为核心实验工具，涉及DNA损伤应答机制及疾病相关性研究。如需具体文献来源，建议通过抗体供应商(如Santa Cruz Biotechnology)的产品页或PubMed进一步查询。
The p95/NBS1 (Ab-343) antibody is a phospho-specific reagent targeting the NBS1 protein, a critical component of the MRE11-RAD50-NBS1 (MRN) complex involved in DNA damage response and repair. NBS1 (Nijmegen breakage syndrome 1), encoded by the *NBN* gene, is essential for recognizing and signaling double-strand breaks (DSBs). Mutations in NBS1 are linked to Nijmegen breakage syndrome, a chromosomal instability disorder characterized by radiation sensitivity and cancer predisposition. The antibody specifically detects NBS1 phosphorylated at serine 343 (Ser343), a key post-translational modification induced by ATM/ATR kinases following DNA damage. This phosphorylation event facilitates cell cycle checkpoint activation and recruitment of repair factors.
The Ab-343 clone is widely used in research to study DSB repair mechanisms, particularly in contexts like ionizing radiation, chemotherapeutic agents, or replication stress. It is validated for applications such as Western blotting and immunofluorescence to assess NBS1 activation dynamics. Researchers often employ it in combination with DNA-damaging treatments to evaluate phosphorylation-dependent interactions within the MRN complex or its role in stabilizing stalled replication forks. Its specificity for the phosphorylated form makes it valuable for distinguishing active DNA damage signaling from basal NBS1 expression. This antibody has contributed to insights into genomic instability disorders, cancer biology, and therapeutic responses tied to DNA repair pathways.
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