| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2 |
| Entrez GeneID | 1326 |
| WB Predicted band size | Calculated MW: 53 kDa; Observed MW: 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human COT around the phosphorylation site of Thr290. AA range:256-305 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于STAT6(Ab-641)抗体的3-4条参考文献示例(虚构内容，仅作参考)：
1. **文献名称**：*STAT6 Phosphorylation in Th2 Cell Differentiation*
**作者**：Smith et al. (2020)
**摘要**：研究STAT6在Th2淋巴细胞分化中的作用，通过STAT6(Ab-641)抗体检测IL-4诱导的STAT6磷酸化，证实其调控下游基因表达。
2. **文献名称**：*Role of STAT6 in Tumor-Associated Macrophage Polarization*
**作者**：Zhang et al. (2018)
**摘要**：利用STAT6(Ab-641)进行免疫组化和Western blot分析，揭示STAT6信号通路在肿瘤微环境中驱动M2型巨噬细胞极化的机制。
3. **文献名称**：*STAT6 Activation in Allergic Asthma Models*
**作者**：Lee et al. (2019)
**摘要**：通过STAT6(Ab-641)抗体在小鼠哮喘模型中验证STAT6的激活状态，发现其介导嗜酸性粒细胞浸润和气道高反应性。
4. **文献名称**：*STAT6-Dependent Gene Regulation in B Cells*
**作者**：Brown et al. (2021)
**摘要**：使用STAT6(Ab-641)进行染色质免疫共沉淀(ChIP)，揭示STAT6直接结合特定基因启动子区，调控B细胞抗体类别转换。
(注：以上文献为模拟内容，实际引用需查询真实数据库如PubMed。)
The STAT6 (Ab-641) antibody is a widely used research tool designed to detect signal transducer and activator of transcription 6 (STAT6), a transcription factor involved in cytokine-mediated signaling pathways. STAT6 is primarily activated by interleukin-4 (IL-4) and interleukin-13 (IL-13) through phosphorylation at specific tyrosine residues (e.g., Tyr641), leading to its dimerization, nuclear translocation, and regulation of genes critical for immune responses, allergic inflammation, and Th2 cell differentiation. The antibody, often raised in rabbits or mice as a monoclonal or polyclonal reagent, specifically recognizes phosphorylated or total STAT6 protein, depending on its design.
STAT6 (Ab-641) is commonly validated for applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Researchers employ it to study STAT6’s role in diseases such as asthma, atopic dermatitis, and certain cancers (e.g., lymphoma), where aberrant STAT6 activation is implicated. The antibody’s specificity is typically confirmed using knockout cell lines or peptide competition assays. Its utility extends to both basic research and preclinical studies, particularly in exploring therapeutic targets that modulate IL-4/IL-13-STAT6 signaling. Proper validation ensures minimal cross-reactivity with other STAT family members, making it a reliable tool for dissecting STAT6-dependent molecular mechanisms.
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