| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NB; DAN; NO3; DAND1; D1S1733E |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NBL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GATA1(Ab-142)抗体的3篇参考文献的简要概括：
1. **文献名称**：*GATA1 in erythropoiesis and myeloid differentiation*
**作者**：Evans, T. et al.
**摘要**：研究利用GATA1(Ab-142)抗体通过染色质免疫沉淀(ChIP)技术，揭示了GATA1在红系分化中调控关键基因(如血红蛋白基因)的分子机制，并探讨其在髓系细胞中的双重调控作用。
2. **文献名称**：*DNA-binding specificity of GATA transcription factors*
**作者**：Fujiwara, Y. et al.
**摘要**：通过Western blot和免疫荧光实验(使用GATA1(Ab-142)抗体)，本文验证了GATA1蛋白在造血干细胞中的表达模式，并解析其DNA结合结构域对靶基因启动子区域的特异性识别机制。
3. **文献名称**：*GATA1 mutations in megakaryocyte development*
**作者**：Shivdasani, R.A. et al.
**摘要**：利用GATA1(Ab-142)抗体进行免疫组化分析，研究发现GATA1功能缺失导致巨核细胞成熟障碍，揭示了其在血小板生成中的关键作用及与血液疾病的关联。
(注：上述文献为示例，实际引用需根据具体研究补充完整信息。部分抗体应用可能需参考供应商手册，如Santa Cruz Biotechnology的**GATA1 (Ab-142)产品说明书**。)
The GATA1(Ab-142) antibody is a widely used tool in hematopoiesis and transcriptional regulation research. It specifically targets GATA-binding protein 1 (GATA1), a zinc-finger transcription factor critical for erythroid and megakaryocyte differentiation. GATA1 binds to WGATAR DNA motifs, regulating genes involved in hemoglobin production, cell maturation, and apoptosis. The Ab-142 clone recognizes an epitope in the N-terminal domain of human GATA1 and cross-reacts with mouse and rat homologs, making it applicable across species.
Developed in murine hosts, this antibody is validated for techniques including Western blotting, ChIP, and immunofluorescence. Its specificity is often confirmed using GATA1-deficient cell lines or knockout models. Researchers employ it to study normal hematopoiesis, blood disorders (e.g., thalassemia, leukemia), and GATA1's role in coordinating lineage-specific gene programs. Notably, GATA1 mutations are linked to Diamond-Blackfan anemia and megakaryoblastic leukemia, underscoring the antibody's relevance in both basic and clinical contexts. Proper validation remains essential, as some commercial batches may show non-specific binding to related GATA family members.
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