| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ELK1; ETS-domain protein Elk-1; |
| Entrez GeneID | 2002; |
| WB Predicted band size | 62kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.387~391 (P-R-S-P-A) derived from Human Elk1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Elk1(Ab-389)抗体的3篇示例参考文献(注：内容为模拟生成，实际文献需通过学术数据库查询)：
1. **文献名称**：*"Phosphorylation-dependent regulation of Elk-1 transcriptional activity in neuronal differentiation"*
**作者**：Smith J, et al.
**摘要**：研究Elk1在神经元分化中的转录调控机制，通过Elk1(Ab-389)抗体检测其磷酸化状态(Ser389位点)，发现磷酸化Elk1通过激活靶基因促进突触可塑性。
2. **文献名称**：*"Elk1-mediated gene expression in cancer progression: Insights from ChIP-seq analysis"*
**作者**：Chen L, et al.
**摘要**：利用Elk1(Ab-389)抗体进行染色质免疫沉淀(ChIP-seq)，揭示Elk1在乳腺癌细胞中结合特定基因启动子区域，调控肿瘤侵袭相关基因表达。
3. **文献名称**：*"Validation of a specific Elk1 antibody (Ab-389) for Western blot and immunofluorescence applications"*
**作者**：Patel R, et al.
**摘要**：验证Elk1(Ab-389)抗体的特异性，证明其在Western blot中识别90kDa Elk1蛋白，并在免疫荧光实验中定位Elk1于细胞核，为后续功能研究提供工具支持。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“Elk1 antibody Ab-389”或“Elk1 Ser389 phosphorylation”获取最新研究。
**Elk1 (Ab-389) Antibody Background**
The Elk1 (Ab-389) antibody is a monoclonal or polyclonal immune reagent specifically designed to detect Elk1. a transcription factor belonging to the ETS (E26 transformation-specific) family. Elk1 plays a critical role in regulating gene expression by binding to serum response elements (SREs) in promoter regions, often in conjunction with serum response factor (SRF). It is activated through phosphorylation by mitogen-activated protein kinases (MAPKs), such as ERK, JNK, and p38. in response to extracellular signals like growth factors, stress, or cytokines. This activation enables Elk1 to mediate cellular processes including proliferation, differentiation, and apoptosis.
The antibody (Ab-389) typically targets a specific epitope or domain within Elk1. such as its N-terminal transactivation domain or C-terminal ETS DNA-binding domain, depending on the immunogen design. It is widely used in techniques like Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence to study Elk1 expression, localization, and interaction with DNA or partner proteins.
Elk1 dysregulation has been implicated in cancer, neurological disorders, and cardiovascular diseases, making this antibody a valuable tool for both basic research and translational studies. Validated for specificity and sensitivity, Elk1 (Ab-389) is often referenced in studies exploring MAPK signaling pathways, immediate-early gene activation, and transcriptional networks. Commercial suppliers typically provide detailed validation data, including reactivity across species (e.g., human, mouse, rat) and applications.
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