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Rabbit Polyclonal Myc(Ab-358) Antibody

  • 中文名: Myc(Ab-358)抗体
  • 别    名: c-myc
货号: IPDX41267
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Aliasesc-myc
Entrez GeneID4609;
WB Predicted band size60kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPeptide sequence around aa.356~360 (R-R-T-H-N) derived from Human Myc.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于Myc (Ab-358)抗体的假设性参考文献示例(仅供参考,实际文献需进一步验证):

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1. **文献名称**: *"c-Myc regulation of cell proliferation and apoptosis in cancer"*

**作者**: Smith, J. et al.

**摘要**: 研究c-Myc在肿瘤细胞增殖和凋亡中的作用,使用Myc (Ab-358)抗体进行Western blot和免疫组化分析,验证其在多种癌细胞系中的表达水平。

2. **文献名称**: *"Epitope-specific monoclonal antibody generation for c-Myc detection"*

**作者**: Lee, H. & Kim, S.

**摘要**: 描述了Myc (Ab-358)抗体的开发与验证,通过ELISA和免疫沉淀实验证明其特异性识别c-Myc蛋白的特定表位,适用于ChIP-seq和免疫荧光技术。

3. **文献名称**: *"MYC-driven metabolic reprogramming in glioblastoma"*

**作者**: Zhang, Y. et al.

**摘要**: 探讨MYC蛋白在胶质母细胞瘤代谢调控中的作用,使用Ab-358抗体进行免疫印迹和流式细胞术,证实其在高MYC表达模型中的可靠性。

4. **文献名称**: *"A high-throughput screen for MYC-binding proteins using Ab-358-based immunoprecipitation"*

**作者**: Brown, R. et al.

**摘要**: 基于Myc (Ab-358)抗体的免疫沉淀结合质谱分析,鉴定了多个与MYC相互作用的蛋白,揭示了其在转录调控网络中的新功能。

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**建议**:

- 可通过PubMed或Google Scholar搜索 **“Myc Ab-358 antibody”** 或 **“c-Myc antibody applications”** 获取真实文献。

- 查看抗体供应商(如Abcam、CST等)的产品说明书,通常列有引用该抗体的研究。

- 若具体编号“Ab-358”难以匹配,可能需扩大关键词范围至“c-Myc抗体”相关研究。

背景信息

The Myc(Ab-358) antibody is a widely used tool in molecular biology and cancer research, specifically targeting the Myc family of transcription factors, which includes c-Myc, N-Myc, and L-Myc. These proteins regulate genes involved in cell proliferation, differentiation, apoptosis, and metabolism. Dysregulation of Myc proteins is strongly linked to oncogenesis, making them critical targets in cancer studies. The Myc(Ab-358) antibody is designed to recognize a specific phosphorylation site (Tyr358) or epitope within the Myc protein, depending on the manufacturer's design, though precise details may vary. It is commonly employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect endogenous or overexpressed Myc proteins in cell lines, tissues, or engineered models. This antibody is particularly valuable for investigating Myc protein dynamics, post-translational modifications, and interactions in signaling pathways. Its specificity is often validated in knockout or knockdown systems to ensure minimal cross-reactivity. Researchers use Myc(Ab-358) to explore Myc's role in tumor progression, stem cell biology, and therapeutic resistance. Commercial suppliers typically provide data on its reactivity across species (e.g., human, mouse, rat) and applications, with optimization guidelines for experimental conditions. Proper controls, such as isotype-matched antibodies and Myc-deficient samples, are recommended to confirm signal authenticity.

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