| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-binding factor KBF1; EBP-1; NF-kappa-B1 p84/NF-kappa-B1 p98; NFKB1; NFkB-p50 |
| Entrez GeneID | 4790; |
| WB Predicted band size | 50 120 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.335~339 (R-K-S-D-L) derived from Human NFkB-p105. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NF-κB p105/p50(Ab-337)抗体的假设性参考文献示例，基于典型研究领域和常见作者整理：
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1. **文献名称**：*"NF-κB p50 Phosphorylation and Proteasomal Processing of p105 in TLR4 Signaling"*
**作者**：Karin M, Ben-Neriah Y
**摘要**：本研究阐明了TLR4激活后p105的磷酸化机制及其通过蛋白酶体加工为p50的过程，利用Ab-337抗体通过免疫印迹和免疫沉淀验证p50的生成及功能。
2. **文献名称**：*"Role of p50 in Regulating Inflammatory Gene Expression in Macrophages"*
**作者**：Ghosh S, Hayden MS
**摘要**：通过Ab-337抗体进行染色质免疫共沉淀(ChIP)和免疫荧光实验，揭示p50亚基在巨噬细胞中抑制促炎细胞因子表达的调控作用。
3. **文献名称**：*"Dysregulation of NF-κB p50 in Colorectal Cancer Progression"*
**作者**：Baldwin AS Jr, Wu L
**摘要**：该研究利用Ab-337抗体进行免疫组化分析，发现p50在结直肠癌组织中异常核定位，并与患者预后不良相关，提示其作为潜在生物标志物的价值。
4. **文献名称**：*"Antibody-Specific Validation for NF-κB p50 in Autoimmune Disease Models"*
**作者**：Li Q, Verma IM
**摘要**：评估Ab-337抗体在小鼠自身免疫模型中的特异性，通过Western blot和流式细胞术证实其在p50检测中的高灵敏度和低交叉反应性。
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**说明**：以上文献为示例性内容，实际引用时需根据具体研究通过学术数据库(如PubMed、Web of Science)检索并核实。若需真实文献，可尝试搜索关键词“NF-κB p50 antibody Ab-337”或联系抗体供应商获取相关引用资料。
The NF-κB-p105/p50 (Ab-337) antibody is designed to detect specific isoforms of the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) transcription factor family, particularly targeting the p105 precursor and its processed subunit p50. NF-κB proteins play pivotal roles in regulating immune responses, inflammation, cell survival, and proliferation by controlling the expression of genes involved in these processes. The p105 protein (encoded by the NFKB1 gene) serves as both a precursor for p50 and an IκB-like inhibitor, retaining NF-κB complexes in the cytoplasm under basal conditions. Proteasomal processing of p105 removes its C-terminal inhibitory domain, generating the mature p50 subunit, which can form homodimers or heterodimers (e.g., with RelA/p65) to modulate DNA binding and transcriptional activity.
The Ab-337 epitope corresponds to phosphorylated serine residues (e.g., Ser337 in human p105/p50), a modification critical for regulating p105 stability, processing, and NF-κB signaling dynamics. This phosphorylation event is often mediated by kinases such as IKK (IκB kinase) in response to stimuli like cytokines, pathogens, or stress. Researchers employ this antibody in applications such as Western blotting, immunoprecipitation, and immunofluorescence to investigate NF-κB activation mechanisms, particularly in contexts like inflammation, immune disorders, and cancer. Its specificity for phosphorylated p105/p50 makes it a valuable tool for studying post-translational regulation of NF-κB pathways and evaluating therapeutic targets in diseases driven by aberrant NF-κB activity.
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