| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Patatin-like phospholipase domain-containing protein 4, Protein GS2, PNPLA4, DXS1283E, GS2 |
| Entrez GeneID | 8228 |
| WB Predicted band size | 28.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PNPLA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the C-terminal region of human PNPLA4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及NF-κB p65(Ser276磷酸化相关)抗体的参考文献示例(内容为模拟概括，实际文献请核实)：
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1. **"Phosphorylation of RelA/p65 on Serine 276 Regulates NF-κB-Driven Gene Expression"**
*作者：Chen L, et al.*
摘要：研究揭示了p65亚基Ser276位点的磷酸化通过招募组蛋白乙酰转移酶CBP/p300.促进染色质重塑和促炎基因(如IL-6、COX-2)的转录激活，采用Ab-276抗体进行ChIP实验验证其与靶基因启动子的结合。
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2. **"MSK1-Mediated Phosphorylation of NF-κB p65 at Ser276 Modulates Cellular Apoptosis"**
*作者：Wang X, et al.*
摘要：利用Ab-276抗体进行Western blot和免疫荧光分析，证明丝裂原活化蛋白激酶MSK1在DNA损伤后磷酸化p65(Ser276)，抑制细胞凋亡并促进生存信号，为癌症治疗提供潜在靶点。
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3. **"TNF-α Induces Cyclin D1 Expression via p65 Ser276 Phosphorylation-Dependent Chromatin Remodeling"**
*作者：Kim HJ, et al.*
摘要：通过Ab-276抗体阻断Ser276磷酸化，发现TNF-α通过IKKβ/MSK1通路激活p65.驱动Cyclin D1表达和细胞周期进程，揭示炎症与肿瘤增殖的分子关联。
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注：以上文献名称及摘要内容为示例性质，实际研究中请根据具体抗体说明书或数据库(如PubMed)检索真实文献。若需精确文献，可提供抗体生产商(如CST #3037)或DOI编号进一步查询。
The NF-κB-p65 (Ab-276) antibody is a widely used tool in molecular biology for studying the NF-κB signaling pathway, a critical regulator of immune responses, inflammation, and cell survival. The p65 subunit, also known as RelA, is a key component of the NF-κB transcription factor family, typically forming heterodimers with p50. This antibody specifically recognizes phosphorylated p65 at serine 276 (Ser276), a post-translational modification that modulates transcriptional activity by enhancing interactions with coactivators like CBP/p300. Phosphorylation at Ser276 is induced by kinases such as MSK1 or PKA in response to stimuli like TNF-α, IL-1β, or LPS, marking pathway activation.
Developed for applications including Western blotting, immunoprecipitation, immunofluorescence, and ChIP, the Ab-276 antibody helps researchers investigate NF-κB dynamics in diseases like cancer, autoimmune disorders, and chronic inflammation. Its specificity for the phosphorylated epitope allows precise tracking of activated NF-κB in cell lines, tissues, or primary samples. Validation often includes knockout controls or phosphorylation-blocking peptides to confirm target specificity. Commercial suppliers (e.g., Cell Signaling Technology, Santa Cruz Biotechnology) provide detailed protocols for optimal use. Understanding p65 phosphorylation provides insights into therapeutic strategies targeting NF-κB dysregulation.
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