Rabbit Polyclonal GSK3a(Ab-21) Antibody

The GSK3α (Ab-21) antibody is a widely used tool in biomedical research for detecting Glycogen Synthase Kinase 3 alpha (GSK3α), a serine/threonine kinase involved in critical cellular processes. GSK3α, along with its isoform GSK3β, regulates signaling pathways such as Wnt, Hedgehog, and insulin signaling, impacting metabolism, cell proliferation, and differentiation. Unlike GSK3β, GSK3α contains a unique glycine-rich N-terminal region and exhibits distinct substrate interactions. The Ab-21 clone specifically recognizes endogenous GSK3α, often targeting epitopes near regulatory phosphorylation sites. For example, phosphorylation at Ser21 (by kinases like AKT) inhibits GSK3α activity, altering substrate binding and modulating cellular responses. This antibody is commonly employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study GSK3α expression, localization, and post-translational modifications in diseases such as Alzheimer’s, cancer, and diabetes. Its specificity and reliability make it valuable for elucidating GSK3α's role in pathological mechanisms and therapeutic targeting. Researchers frequently validate findings using siRNA knockdown or kinase inhibitors to confirm functional correlations. The antibody's utility extends across cell lines, tissues, and animal models, supporting its role in both basic and translational studies of GSK3α-associated pathways.