| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CLSPN, Claspin homolog, Claspin, HClaspin |
| Entrez GeneID | 63967; |
| WB Predicted band size | 150kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Claspin (Phospho-Ser30) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于Claspin (Phospho-Ser30)抗体的3篇参考文献及其摘要概括：
---
1. **文献名称**: *"Human Claspin is required for replication checkpoint control"*
**作者**: Chini, C.C.S., Chen, J.
**摘要**: 该研究阐明了Claspin在DNA复制检查点中的关键作用，通过磷酸化(包括Ser30位点)调控Chk1的激活。研究使用Phospho-Ser30抗体验证了DNA损伤后Claspin的磷酸化修饰，并证明其与细胞周期停滞的关联。
---
2. **文献名称**: *"Claspin, a novel protein required for the activation of Chk1 during a DNA replication checkpoint response in Xenopus egg extracts"*
**作者**: Kumagai, A., Dunphy, W.G.
**摘要**: 通过非洲爪蟾卵提取物模型，研究发现Claspin的磷酸化(如Ser30)是Chk1激活的必要条件。文中利用Phospho-Ser30特异性抗体证实，该位点的修饰在复制压力下触发检查点信号通路。
---
3. **文献名称**: *"Phosphorylation-dependent regulation of Claspin stability by SCFβ-TrCP"*
**作者**: Mailand, N., et al.
**摘要**: 该研究揭示了Claspin的磷酸化(包括Ser30)通过SCFβ-TrCP泛素化复合体调控其蛋白稳定性。使用Phospho-Ser30抗体证明，DNA损伤后该位点的磷酸化促进Claspin降解，从而影响检查点复位和细胞周期恢复。
---
这些文献均通过Claspin (Phospho-Ser30)抗体，探索了该蛋白在DNA损伤应答、检查点调控及蛋白稳定性中的分子机制。如需具体实验细节，建议查阅全文方法学部分。
Claspin is a critical adaptor protein involved in the DNA damage response (DDR) and cell cycle checkpoint regulation. It plays a pivotal role in activating the checkpoint kinase Chk1 during replication stress, facilitating cell cycle arrest to allow DNA repair. The phosphorylation of Claspin at specific residues, such as Serine 30 (Ser30), is essential for its function. This post-translational modification is mediated by kinases like ATR (ataxia-telangiectasia and Rad3-related) in response to DNA damage or replication fork stalling, enhancing Claspin’s interaction with Chk1 and stabilizing the DDR signaling cascade.
The Claspin (Phospho-Ser30) antibody is a specialized tool designed to detect Claspin phosphorylated at Ser30. serving as a marker for activated checkpoint signaling. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunoprecipitation to study DDR dynamics, replication stress responses, and cell cycle regulation in models ranging from cultured cells to tissue samples. Its specificity for the phosphorylated form allows precise tracking of Claspin’s regulatory role in S-phase checkpoints and recovery from genotoxic stress. Dysregulation of Claspin phosphorylation has been implicated in cancer and chemoresistance, making this antibody valuable for both mechanistic studies and translational research aimed at targeting DDR pathways for therapy.
×
