Rabbit Polyclonal QIK/QSK(Phospho-Thr175/221) Antibody

The QIK/QSK (Phospho-Thr175/221) antibody is designed to detect phosphorylated forms of specific threonine residues (Thr175 and Thr221) in target proteins, often associated with signaling pathways regulating cell proliferation, stress responses, or apoptosis. These phosphorylation sites are critical for modulating protein activity, conformation, or interactions with downstream effectors. For example, in certain kinases or regulatory proteins, phosphorylation at Thr175/221 may activate catalytic function or trigger conformational changes required for substrate binding. The antibody is commonly used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to study dynamic phosphorylation events under conditions such as DNA damage, metabolic stress, or growth factor stimulation. Specificity validation typically involves knockout cell lines, phosphopeptide competition assays, or phosphatase treatment to confirm signal dependence on the phosphorylated epitope. Research applications span cancer biology (e.g., tumor suppressor pathways), neuroscience (e.g., synaptic plasticity), and immunology (e.g., inflammatory signaling). Proper experimental controls are essential due to potential cross-reactivity with structurally similar phosphorylated motifs in related proteins.