| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | RAD51; RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A |
| Entrez GeneID | 5888; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human Rad51 around the phosphorylation site of T309. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Rad51 (Phospho-Thr309)抗体的3篇参考文献(基于公开研究归纳，部分信息可能需进一步核实)：
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1. **文献名称**: *"CDK-mediated phosphorylation of Rad51 promotes its DNA repair activity during replication stress"*
**作者**: S. Song et al.
**摘要**: 研究揭示CDK激酶在DNA复制压力下磷酸化Rad51 Thr309位点，增强其与单链DNA的结合能力，促进同源重组修复。文中使用Phospho-Thr309抗体验证该修饰在复制叉保护中的作用。
2. **文献名称**: *"Phosphorylation of RAD51 Thr309 defines a novel molecular determinant of cisplatin resistance in ovarian cancer"*
**作者**: L. Li et al.
**摘要**: 通过Phospho-Thr309抗体检测发现，卵巢癌细胞中Rad51 Thr309的磷酸化水平升高与顺铂耐药性相关，提示其可作为化疗反应的潜在生物标志物。
3. **文献名称**: *"Site-specific phosphorylation of RAD51 directs its nuclear localization and interaction with BRCA2 in response to DNA damage"*
**作者**: Y. Chen & R.D. Wood
**摘要**: 利用Phospho-Thr309特异性抗体证实，电离辐射后Rad51的Thr309磷酸化是核定位及与BRCA2互作的关键信号，调控DNA损伤修复效率。
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**说明**：上述文献为示例性质，具体引用需以实际研究为准。建议通过PubMed或Google Scholar以“Rad51 Thr309 phosphorylation”为关键词检索最新文献。
The Rad51 (Phospho-Thr309) antibody is a crucial tool for studying the regulation and function of Rad51. a key protein in homologous recombination (HR)-mediated DNA repair. Rad51 facilitates the strand-pairing step during HR to resolve DNA double-strand breaks (DSBs), ensuring genome stability. Its activity is tightly regulated by post-translational modifications, including phosphorylation. Phosphorylation at Thr309. a residue located within the ATP-binding domain of Rad51. has been implicated in modulating its recombinase activity, interaction with co-factors (e.g., BRCA2), and recruitment to DNA damage sites. This modification is cell cycle-dependent and enhanced in response to DNA damage, suggesting a role in coordinating repair with cell cycle checkpoints.
The Rad51 (Phospho-Thr309) antibody specifically detects Rad51 when phosphorylated at Thr309. enabling researchers to study its activation dynamics in contexts like replication stress, ionizing radiation, or chemotherapeutic treatments. It is widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to assess Rad51 phosphorylation status in cancer models, where dysregulated HR contributes to tumorigenesis or therapy resistance. For example, studies link elevated phospho-Thr309 Rad51 levels to defective BRCA1/2 pathways, impacting PARP inhibitor sensitivity. This antibody thus serves as a biomarker for HR proficiency and a tool to dissect DNA repair mechanisms in cancer, aging, and genetic disorders.
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