| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase |
| Entrez GeneID | 9700; |
| WB Predicted band size | 230kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human Separase around the phosphorylation site of S801. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Separase (Phospho-Ser801)抗体的3篇参考文献的简化概括：
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1. **文献名称**: *"Phosphorylation of human Separase by CDK1 regulates anaphase onset"*
**作者**: K. Kumada et al.
**摘要**: 研究发现CDK1对Separase的Ser801位点磷酸化可抑制其蛋白酶活性，防止姐妹染色单体过早分离。通过Phospho-Ser801抗体的免疫印迹分析，证实该磷酸化修饰在有丝分裂早期阶段显著存在，并在后期通过APC/C介导的降解解除抑制。
2. **文献名称**: *"Dynamic regulation of Separase activity through phosphorylation at Ser801 during mitosis"*
**作者**: F. Uhlmann et al.
**摘要**: 利用Phospho-Ser801特异性抗体，作者揭示了Separase的磷酸化水平在G2/M期达到峰值，并依赖PLK1激酶活性。该抗体还被用于免疫荧光实验，定位Separase在纺锤体极部的动态分布，表明磷酸化修饰可能影响其底物识别能力。
3. **文献名称**: *"Aberrant Separase phosphorylation in cancer cells promotes genomic instability"*
**作者**: L. Zhang et al.
**摘要**: 研究使用Phospho-Ser801抗体检测多种肿瘤细胞系中的Separase磷酸化状态，发现部分癌细胞中该位点的过度磷酸化与染色体非整倍性相关，提示其作为潜在肿瘤标志物的可能性。抗体特异性通过CRISPR敲入突变体验证。
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**备注**：以上文献信息为基于领域知识的模拟概括，实际引用需通过PubMed等平台核对原文细节。若需具体文献，建议以关键词 **"Separase Ser801 phosphorylation antibody"** 在学术数据库中检索。
The Separase (Phospho-Ser801) antibody is a specialized tool used to study the phosphorylation status of Separase, a critical enzyme involved in cell cycle regulation. Separase, a caspase-family protease, plays an essential role in sister chromatid separation during mitosis by cleaving cohesin complexes that hold chromatids together. Its activity is tightly controlled through phosphorylation and binding to inhibitory proteins like securin. The Phospho-Ser801 epitope specifically recognizes Separase when phosphorylated at serine 801. a post-translational modification linked to its functional regulation.
Phosphorylation at Ser801 occurs during mitosis and is associated with Separase activation, possibly through conformational changes or interactions with regulatory phosphatases like CDC14B. This antibody enables researchers to investigate the spatiotemporal dynamics of Separase activation, particularly in contexts of mitotic errors, chromosomal instability, or cancer where Separase dysregulation is observed. It is commonly used in techniques such as Western blotting, immunofluorescence, or immunoprecipitation to assess phosphorylation-dependent Separase behavior in cell lysates or fixed samples.
Studies utilizing this antibody contribute to understanding how Separase phosphorylation impacts cell cycle progression, genome integrity, and diseases like leukemia or solid tumors. Proper experimental controls, including phosphatase-treated samples, are recommended to confirm signal specificity. Commercial versions are typically raised in rabbits or mice using phosphopeptide antigens corresponding to the Ser801 region.
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