| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | PPP1R12A; MBS; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit |
| Entrez GeneID | 4659; |
| WB Predicted band size | 130kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human MYPT1 around the phosphorylation site of T853. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于MYPT1 (Phospho-Thr853)抗体的参考文献，按文献名称、作者和摘要内容简要整理：
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1. **文献名称**：*Phosphorylation of the myosin phosphatase targeting subunit by Rho-kinase*
**作者**：Feng J, Ito M, Ichikawa K, et al.
**摘要**：研究Rho激酶(ROCK)对MYPT1 Thr853位点的磷酸化作用，发现其通过抑制肌球蛋白磷酸酶活性增强平滑肌收缩。文中使用Phospho-Thr853抗体验证该位点的磷酸化与Rho信号通路的直接关联。
2. **文献名称**：*Rho-associated kinase directly induces smooth muscle contraction through myosin light chain phosphorylation and interaction with myosin phosphatase*
**作者**：Kitazawa T, Masuo M, Somlyo AP.
**摘要**：探讨Rho激酶通过磷酸化MYPT1 Thr853位点调控肌球蛋白磷酸酶活性，从而介导平滑肌收缩。研究通过特异性抗体证实Thr853磷酸化在收缩信号传导中的关键作用。
3. **文献名称**：*Inhibition of myosin phosphatase by upregulated Rho-kinase: a key mechanism underlying vascular hypercontractility in hypertension*
**作者**：Murthy KS, Zhou H, Grider JR, et al.
**摘要**：在高血压模型中，Rho激酶活性上调导致MYPT1 Thr853磷酸化水平升高，抑制磷酸酶活性并增强血管收缩。研究利用Phospho-Thr853抗体证明该位点磷酸化与病理状态的相关性。
4. **文献名称**：*Phosphorylation of Thr853 is essential for the regulation of myosin phosphatase by protein kinase C-potentiated inhibitor protein*
**作者**：Eto M, Kitazawa T, Brautigan DL.
**摘要**：揭示Thr853磷酸化是调控肌球蛋白磷酸酶活性的关键机制，并通过特异性抗体验证该位点磷酸化在PKC信号通路中的功能重要性。
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这些文献均涉及MYPT1 Thr853磷酸化的分子机制及其在生理/病理过程中的作用，抗体应用明确且结论具有代表性。
The MYPT1 (Myosin Phosphatase Target Subunit 1) protein is a regulatory subunit of myosin phosphatase, a critical enzyme complex that dephosphorylates myosin light chain (MLC) to regulate smooth muscle and non-muscle cell contraction. Phosphorylation at Thr853 on MYPT1. mediated by kinases such as Rho-associated protein kinase (ROCK), inhibits myosin phosphatase activity, leading to sustained MLC phosphorylation and enhanced cellular contractility. This post-translational modification plays a key role in signaling pathways involving RhoA/ROCK, which are implicated in vascular tone, cell motility, and cytoskeletal organization.
The MYPT1 (Phospho-Thr853) antibody is a specialized tool designed to detect MYPT1 phosphorylated specifically at Thr853. It is widely used in research to investigate the regulatory mechanisms of smooth muscle contraction, endothelial function, and pathological conditions such as hypertension, cardiovascular remodeling, and cancer metastasis. By enabling precise detection of this phosphorylation event via techniques like Western blotting and immunofluorescence, the antibody helps elucidate the dynamic balance between kinase and phosphatase activities in cellular signaling. Its specificity for the phosphorylated form of MYPT1 makes it valuable for studying ROCK pathway activation and evaluating therapeutic inhibitors targeting this pathway.
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