| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GRLF1, P190A, P190-A, p190RhoGAP, p190ARhoGAP |
| Entrez GeneID | 2909; |
| WB Predicted band size | 155kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine 1105 (N-I-Y(p)-S-V) derived from Human GRF-1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GRF-1 (Phospho-Tyr1105)抗体的3篇参考文献的简要概括(注：文献为假设示例，实际需根据真实研究补充)：
1. **文献名称**：*"Phosphorylation of RasGRF1 at Tyr1105 Mediates EGFR-Dependent Ras Activation"*
**作者**：Lee et al.
**摘要**：研究揭示了EGFR信号通过磷酸化RasGRF1的Tyr1105位点激活Ras通路，使用Phospho-Tyr1105抗体验证了该修饰在肿瘤细胞迁移中的作用。
2. **文献名称**：*"GRF-1 Tyrosine 1105 Phosphorylation Regulates Neuronal Differentiation"*
**作者**：Chen & Wang
**摘要**：通过Phospho-Tyr1105抗体检测，发现Tyr1105磷酸化在神经干细胞分化中调控Ras/MAPK信号，影响神经元成熟。
3. **文献名称**：*"Role of RasGRF1 Phosphorylation in Cardiac Hypertrophy Signaling"*
**作者**：Rodriguez et al.
**摘要**：利用Phospho-Tyr1105特异性抗体证实，压力负荷诱导的心肌肥厚与RasGRF1的Tyr1105磷酸化水平升高相关。
建议通过PubMed或Google Scholar以关键词 **"RasGRF1 Tyr1105 phosphorylation antibody"** 查询真实文献，或参考抗体供应商(如CST、Abcam)的产品引用列表。
The GRF-1 (Phospho-Tyr1105) antibody is a specialized tool used to detect the phosphorylated form of GRF-1 (Growth Factor Receptor-Bound Protein 2-Related Adaptor Protein 1) at tyrosine residue 1105. GRF-1. also known as GAB1 or Grap-2. is a key adaptor protein involved in intracellular signaling pathways, particularly those mediated by receptor tyrosine kinases (RTKs). Upon activation of RTKs by growth factors (e.g., EGF, insulin), GRF-1 is recruited to the cell membrane, where it undergoes phosphorylation at specific tyrosine residues, including Tyr1105. This post-translational modification facilitates the assembly of multiprotein complexes, enabling downstream activation of signaling cascades such as the MAPK/ERK and PI3K/AKT pathways, which regulate cell proliferation, survival, and differentiation.
The Phospho-Tyr1105 antibody is designed to specifically recognize GRF-1 only when phosphorylated at this site, making it a critical reagent for studying dynamic signaling events in response to extracellular stimuli. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess GRF-1 activation status, pathway crosstalk, or dysregulation in diseases such as cancer. Its specificity is typically validated using knockout controls or phosphatase-treated samples to confirm the absence of non-phosphorylated cross-reactivity. Proper experimental conditions (e.g., stimulation with growth factors, inhibition of phosphatases) are essential for accurate detection due to the transient nature of phosphorylation events.
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