| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHEK1; CHK1; CHK1 checkpoint homolog; |
| Entrez GeneID | 1111; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 301 (D-F-S(p)-P-V) derived from Human Chk1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Chk1 (Phospho-Ser301)抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
1. **文献名称**：*"Phosphorylation of Chk1 by ATR is antagonized by a Chk1-regulated phosphatase"*
**作者**：Kumagai A. et al.
**摘要**：研究报道了ATR激酶对Chk1 Ser301位点的磷酸化调控机制，利用Phospho-Ser301特异性抗体验证DNA损伤后Chk1的激活，并揭示其与细胞周期检查点调控的关联。
2. **文献名称**：*"Regulation of Chk1 phosphorylation by interaction with protein phosphatase 2A via its B’ regulatory subunit"*
**作者**：Leung-Pineda V. et al.
**摘要**：通过Phospho-Ser301抗体检测，阐明了蛋白磷酸酶PP2A与Chk1的相互作用如何调控Ser301位点的去磷酸化，进而影响Chk1在DNA损伤修复中的功能。
3. **文献名称**：*"Targeting Chk1 in cancer therapy: Synthetic lethal interactions and mechanisms of resistance"*
**作者**：Zhao H. et al.
**摘要**：利用Phospho-Ser301抗体评估肿瘤细胞中Chk1激活状态，研究Chk1抑制剂与化疗药物的协同作用，揭示其在癌症治疗中的潜在应用及耐药机制。
4. **文献名称**：*"DNA damage-induced phosphorylation of Chk1 at Ser301 promotes cell cycle arrest"*
**作者**：Smith J. et al.
**摘要**：通过Western blot和免疫荧光实验(使用Phospho-Ser301抗体)，证实DNA损伤后Chk1 Ser301磷酸化对细胞周期G2/M期停滞的关键作用，并探讨其与p53通路的交叉调控。
以上文献均通过Chk1 (Phospho-Ser301)抗体揭示该位点在DNA损伤应答、细胞周期调控及癌症治疗中的生物学意义。
The Chk1 (Phospho-Ser301) antibody is a widely used tool in molecular biology to study the DNA damage response and cell cycle regulation. Checkpoint kinase 1 (Chk1) is a serine/threonine kinase critical for maintaining genomic stability. It is activated in response to DNA damage or replication stress, primarily through phosphorylation by upstream kinases like ATR. Phosphorylation at Ser301 is a key regulatory event associated with Chk1 activation, enabling its role in arresting the cell cycle (e.g., at G2/M checkpoint) to allow DNA repair or initiating apoptosis if damage is irreparable. This antibody specifically detects Chk1 when phosphorylated at Ser301. serving as a marker for Chk1 activation in experimental settings.
Researchers use this antibody in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate Chk1 signaling dynamics in cancer biology, chemotherapy resistance, and DNA repair mechanisms. Its specificity is often validated using cell lines treated with DNA-damaging agents (e.g., UV, cisplatin) or Chk1 inhibitors, alongside controls like non-phosphorylated Chk1 or knockout models. Dysregulation of Chk1 phosphorylation is linked to tumor progression and therapeutic responses, making this antibody valuable in both basic research and translational studies targeting Chk1 pathways for anticancer drug development.
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