Rabbit Polyclonal JNK1/JNK2/JNK3(phospho-Thr183/Tyr185) Antibody

The c-Jun N-terminal kinase (JNK) family, comprising JNK1 (MAPK8), JNK2 (MAPK9), and JNK3 (MAPK10), is a subgroup of mitogen-activated protein kinases (MAPKs) central to cellular stress responses, apoptosis, inflammation, and differentiation. Activation of JNKs requires dual phosphorylation at conserved Thr183 and Tyr185 residues within the activation loop. Antibodies targeting phospho-Thr183/Tyr185 (p-JNK) are critical tools for detecting activated JNK isoforms in research. These antibodies recognize all three JNK isoforms when phosphorylated at these sites, making them pan-specific for monitoring JNK activation across biological contexts. However, distinguishing between isoforms (JNK1/2 vs. JNK3) often requires additional validation via isoform-specific antibodies or genetic knockdown. JNK1 and JNK2 are ubiquitously expressed, while JNK3 is primarily neuronal. The phosphorylation-dependent activation of JNKs is triggered by upstream kinases (MKK4/7) in response to stressors like UV radiation, cytokines, or oxidative stress. p-JNK antibodies are widely used in Western blotting, immunohistochemistry, and flow cytometry to study signaling dynamics in diseases such as neurodegeneration, cancer, and metabolic disorders. Researchers must validate antibody specificity using knockout controls or phosphatase treatment to confirm phosphorylation-dependent signals. Molecular weight differences (JNK1/2 ~46 kDa, JNK3 ~55 kDa) aid in isoform identification during analysis.