Rabbit Polyclonal TrkB(Phospho-Tyr515) Antibody

The TrkB (Phospho-Tyr515) antibody is a specialized tool used to detect the activated form of tropomyosin receptor kinase B (TrkB), a key receptor tyrosine kinase involved in neurotrophic signaling. TrkB binds brain-derived neurotrophic factor (BDNF) and other neurotrophins, playing critical roles in neuronal survival, synaptic plasticity, and cognitive functions. Upon ligand binding, TrkB undergoes dimerization and autophosphorylation at specific tyrosine residues, including Tyr515. within its intracellular kinase domain. Phosphorylation at Tyr515 serves as a docking site for adaptor proteins like Shc or Grb2. initiating downstream signaling cascades such as PI3K/Akt, MAPK/ERK, and PLCγ pathways. These pathways regulate diverse processes, from cell growth to neurotransmitter modulation. The TrkB (Phospho-Tyr515) antibody specifically recognizes this phosphorylation event, enabling researchers to study TrkB activation status in contexts like neurodegenerative diseases, mood disorders, or cancer. It is widely applied in techniques such as Western blotting, immunohistochemistry, and flow cytometry to explore TrkB's role in pathophysiology or therapeutic responses. Dysregulated TrkB signaling is implicated in Alzheimer’s disease, depression, and tumor progression, making this antibody valuable for mechanistic and translational research. Proper controls are essential to ensure specificity, as cross-reactivity with other phosphorylated tyrosine residues or related kinases may occur.