Rabbit Polyclonal SHP-2(Phospho-Tyr542) Antibody

The SHP-2 (Phospho-Tyr542) antibody detects the phosphorylated form of tyrosine 542 in SHP-2 (Src homology 2 domain-containing protein tyrosine phosphatase-2), a ubiquitously expressed non-receptor protein tyrosine phosphatase encoded by the PTPN11 gene. SHP-2 plays critical roles in cell signaling, including RAS/MAPK, PI3K/AKT, and JAK/STAT pathways, regulating cell proliferation, differentiation, and survival. Phosphorylation at Tyr542 occurs upon activation of receptor tyrosine kinases (RTKs) or cytokine receptors, leading to conformational changes that relieve autoinhibition and enhance SHP-2’s phosphatase activity. This post-translational modification is essential for SHP-2’s role in signal transduction, particularly in growth factor and immune response pathways. Dysregulation of SHP-2 phosphorylation is linked to cancers, Noonan syndrome, and immune disorders. The SHP-2 (Phospho-Tyr542) antibody is widely used in research to study SHP-2 activation status, its interaction with signaling partners (e.g., GRB2), and its involvement in disease mechanisms. It is commonly validated in techniques like Western blotting, immunoprecipitation, and immunofluorescence, often using cell lysates from stimulated or disease-model systems. Specificity is confirmed via phosphorylation-blocking peptides or phosphatase treatment.