| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 70Z-SHP; HCP; HCPH; Hematopoietic cell protein-tyrosine phosphatase; PTN6 |
| Entrez GeneID | 5777; |
| WB Predicted band size | 68kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 536 (S-E-Y(p)-G-N) derived from Human SHP-1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SHP-1(Phospho-Tyr536)抗体的示例参考文献(注：以下为构造示例，建议通过PubMed等数据库核实真实文献)：
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1. **"Regulation of SHP-1 tyrosine phosphatase activity by phosphorylation at Tyr536 in T cell signaling"**
*Authors: Lorenz U, Smith J, et al.*
**摘要**：研究SHP-1在T细胞活化中的调控机制，使用Phospho-Tyr536抗体验证其磷酸化状态，发现Tyr536磷酸化抑制SHP-1酶活性，影响下游信号传导。
2. **"Phosphorylation of SHP-1 at Tyr536 modulates its interaction with inhibitory receptors in cancer cells"**
*Authors: Zhang L, Chen H, et al.*
**摘要**：通过免疫沉淀和Western blot(使用Phospho-Tyr536抗体)证明，肿瘤微环境中SHP-1的Tyr536磷酸化增强其与PD-1受体的结合，促进免疫逃逸。
3. **"Role of SHP-1 phosphorylation in cytokine-induced JAK-STAT pathway regulation"**
*Authors: Huang R, Wang Y, et al.*
**摘要**：利用Phospho-Tyr536抗体检测巨噬细胞中SHP-1磷酸化水平，发现IL-6刺激后Tyr536位点磷酸化负反馈调节STAT3激活。
4. **"SHP-1 Tyr536 phosphorylation is required for B cell receptor-mediated apoptosis"**
*Authors: Pao LI, et al.*
**摘要**：在B细胞中通过Phospho-Tyr536抗体分析发现，抗原受体激活诱导的Tyr536磷酸化促进SHP-1募集至BCR复合体，触发凋亡信号。
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**建议**：可通过PubMed、Google Scholar搜索关键词“SHP-1 Tyr536 phosphorylation”或抗体品牌(如CST、Abcam)的产品引用文献获取真实数据。
The SHP-1 (Phospho-Tyr536) antibody is designed to detect the phosphorylated form of SHP-1 (Src homology region 2 domain-containing phosphatase-1) at tyrosine residue 536. SHP-1. encoded by the PTPN6 gene, is a non-receptor protein tyrosine phosphatase (PTP) predominantly expressed in hematopoietic cells. It plays critical roles in regulating immune signaling, cell proliferation, and apoptosis by dephosphorylating target proteins, including receptors (e.g., cytokine receptors) and kinases involved in JAK/STAT, MAPK, and other pathways.
Phosphorylation at Tyr536. located in the C-terminal region, is a key regulatory mechanism for SHP-1 activity. This post-translational modification modulates the phosphatase’s conformation, influencing its catalytic activity or interactions with substrates. Studies suggest that phosphorylation at Tyr536 may either enhance or suppress SHP-1 function depending on cellular context, impacting immune response regulation and disease states such as cancer, autoimmune disorders, and inflammation. The SHP-1 (Phospho-Tyr536) antibody is widely used in immunoblotting (WB), immunoprecipitation (IP), and immunohistochemistry (IHC) to study SHP-1 activation dynamics in signaling pathways. Its application aids in understanding SHP-1’s role in pathological processes and potential therapeutic targeting, particularly in diseases linked to dysregulated tyrosine phosphorylation.
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