| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SH2 domain protein C1; SHC; SHC-transforming protein 1; SHCA; Src homology 2 domain-containing-transforming protein C1 |
| Entrez GeneID | 6464; |
| WB Predicted band size | 46 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N derived from Human Shc1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于Shc1 (Phospho-Tyr427)抗体的3篇参考文献示例，基于典型研究领域和抗体应用场景构建：
---
1. **文献名称**：*"Phosphorylation of Shc1 at Tyr427 regulates EGF-induced MAPK activation and cell proliferation"*
**作者**：Smith A, et al.
**摘要**：本研究揭示了Shc1在Tyr427位点的磷酸化对EGF受体信号传导的关键作用。通过使用特异性Shc1 (Phospho-Tyr427)抗体进行免疫印迹和免疫荧光实验，作者发现该位点的磷酸化是Ras/MAPK通路活化的必要条件，并促进肿瘤细胞的增殖。
2. **文献名称**：*"Role of Shc1 tyrosine phosphorylation in oxidative stress-induced apoptosis"*
**作者**：Chen L, et al.
**摘要**：文章探讨了氧化应激下Shc1的酪氨酸磷酸化动态。利用Phospho-Tyr427抗体，作者证实该位点的磷酸化增强了Shc1与Grb2的相互作用，进而调控线粒体依赖性凋亡通路，影响神经元细胞的存活。
3. **文献名称**：*"Development and validation of a phospho-specific antibody for Shc1 Tyr427 in breast cancer models"*
**作者**：Wang X, et al.
**摘要**：本研究开发并验证了一种高特异性Shc1 (Phospho-Tyr427)抗体。通过质谱和点突变实验确认其特异性后，该抗体被用于临床样本分析，结果显示Tyr427磷酸化水平与乳腺癌的转移和不良预后显著相关。
---
**说明**：以上文献为示例性构造内容，实际文献需通过PubMed、Web of Science等数据库检索关键词(如“Shc1 pY427”、“Shc1 phosphorylation Tyr427”)获取。建议结合具体研究需求筛选近年高质量论文。
The Shc1 (Phospho-Tyr427) antibody is a specialized tool used to detect the phosphorylated form of the Shc1 protein at tyrosine residue 427. Shc1 (Src homology 2 domain-containing transforming protein 1) is an adaptor protein critical for intracellular signaling pathways, particularly those mediated by receptor tyrosine kinases (RTKs). Upon activation of RTKs (e.g., EGFR, Insulin receptor), Shc1 is recruited to phosphorylated receptors via its phosphotyrosine-binding (PTB) domain. Subsequent phosphorylation at Tyr427 creates a docking site for the Grb2-SOS complex, initiating the Ras/MAPK signaling cascade, which regulates cell proliferation, differentiation, and survival.
Phosphorylation of Tyr427 is a key regulatory event in Shc1 signaling. The Shc1 (Phospho-Tyr427) antibody specifically recognizes this post-translational modification, enabling researchers to study Shc1 activation dynamics in response to extracellular stimuli or pathological conditions. It is widely employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess Shc1 activity in cell lines, tissues, or disease models.
This antibody has significant applications in cancer research, as dysregulated Shc1 signaling is implicated in tumor growth, metastasis, and therapy resistance. It also serves as a biomarker for studying metabolic disorders and neurodegenerative diseases linked to RTK/MAPK pathway aberrations. Proper controls (e.g., unphosphorylated Shc1. phosphatase-treated samples) are essential to ensure specificity in experimental workflows.
×
