| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-binding factor KBF1; EBP-1; NF-kappa-B1 p84/NF-kappa-B1 p98; NFKB1; NFkB-p50 |
| Entrez GeneID | 4790; |
| WB Predicted band size | 120 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 927 (C-D-S(p)-G-V) derived from Human NFkB-p105. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NF-κB-p105(Phospho-Ser927)抗体的参考文献示例：
1. **文献名称**：*Phosphorylation of NF-κB p105 by IKKβ regulates subcellular localization and DNA binding*
**作者**：Salmeron A, et al.
**摘要**：研究揭示了IKKβ对p105的Ser927位点磷酸化调控其核转位和DNA结合能力，影响NF-κB的激活及炎症反应。
2. **文献名称**：*Role of Ser927 phosphorylation in the nuclear retention of NF-κB p105*
**作者**：Lang V, et al.
**摘要**：该文献通过抗体检测发现，Ser927磷酸化阻碍p105的蛋白酶体降解，促进其核内滞留并抑制下游靶基因表达。
3. **文献名称**：*MAPK-mediated phosphorylation of NF-κB p105 at Ser927 controls endotoxin tolerance*
**作者**：Bohuslav J, et al.
**摘要**：研究利用Phospho-Ser927抗体证明，LPS诱导的MAPK信号通过该位点磷酸化调控免疫耐受，影响巨噬细胞炎症反应。
4. **文献名称**：*Site-specific phosphorylation of NF-κB p105 by the ubiquitin-like domain kinase TBK1*
**作者**：Buss H, et al.
**摘要**：文献发现TBK1直接磷酸化p105的Ser927.阻断其降解为p50.揭示了其在抗病毒天然免疫中的新机制。
(注：以上文献为示例，实际引用时需核实具体信息。)
The NF-κB-p105 (Phospho-Ser927) antibody is a specialized tool used to detect the phosphorylated form of the NF-κB1 p105 subunit at serine residue 927. NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a family of transcription factors critical for regulating immune responses, inflammation, cell survival, and proliferation. The p105 precursor protein serves dual roles: it acts as a precursor for the p50 subunit (generated via proteasomal processing) and functions as an IκB-like inhibitor by retaining associated NF-κB subunits, such as RelA or c-Rel, in the cytoplasm. Phosphorylation at Ser927. mediated by the IκB kinase (IKK) complex, is a key regulatory event that marks p105 for ubiquitination and partial degradation, liberating active p50 or p105/p50 complexes to translocate into the nucleus and modulate target gene expression.
This antibody is particularly valuable in studies investigating NF-κB activation pathways, such as those triggered by cytokines (e.g., TNF-α, IL-1), pathogens, or stress signals. It enables researchers to assess the phosphorylation status of p105. which correlates with pathway activation and downstream transcriptional activity. Validated for applications like Western blotting, immunoprecipitation, and immunofluorescence, the antibody helps dissect the dynamics of NF-κB signaling in contexts like inflammation, cancer, and immune disorders. Its specificity for the phosphorylated Ser927 epitope ensures accurate detection of the active, processed form of p105. making it a critical reagent for mechanistic and therapeutic research.
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